Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22083 | 3' | -54.6 | NC_004927.1 | + | 2180 | 0.67 | 0.782502 |
Target: 5'- gCGAACCuCGugAGcuaagCUCaCGGUggagUGGUCg -3' miRNA: 3'- -GUUUGG-GCugUCa----GAG-GCCGa---ACCAG- -5' |
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22083 | 3' | -54.6 | NC_004927.1 | + | 4255 | 0.67 | 0.752506 |
Target: 5'- uCGAAUCCacgagaGGCAGUCUCCGGUgucccacaGGUa -3' miRNA: 3'- -GUUUGGG------CUGUCAGAGGCCGaa------CCAg -5' |
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22083 | 3' | -54.6 | NC_004927.1 | + | 7470 | 0.66 | 0.837254 |
Target: 5'- uCGAACCCGgcucccGCAGUCUuuacaCCGccacuuuGCUUGGUa -3' miRNA: 3'- -GUUUGGGC------UGUCAGA-----GGC-------CGAACCAg -5' |
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22083 | 3' | -54.6 | NC_004927.1 | + | 23835 | 0.69 | 0.678858 |
Target: 5'- uGAGCgUCGGCAucGUCUCCGGCgucGUCa -3' miRNA: 3'- gUUUG-GGCUGU--CAGAGGCCGaacCAG- -5' |
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22083 | 3' | -54.6 | NC_004927.1 | + | 53578 | 0.7 | 0.592255 |
Target: 5'- aCAAccuCCCGGCcaaCUCCGGCgagcUGGUCg -3' miRNA: 3'- -GUUu--GGGCUGucaGAGGCCGa---ACCAG- -5' |
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22083 | 3' | -54.6 | NC_004927.1 | + | 58708 | 1.1 | 0.001488 |
Target: 5'- uCAAACCCGACAGUCUCCGGCUUGGUCg -3' miRNA: 3'- -GUUUGGGCUGUCAGAGGCCGAACCAG- -5' |
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22083 | 3' | -54.6 | NC_004927.1 | + | 59394 | 0.66 | 0.820326 |
Target: 5'- cCAAACUCGGCAcacccgagcGUCggCGGCgguaaUGGUCg -3' miRNA: 3'- -GUUUGGGCUGU---------CAGagGCCGa----ACCAG- -5' |
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22083 | 3' | -54.6 | NC_004927.1 | + | 71296 | 0.68 | 0.710895 |
Target: 5'- gGAGCgCGACGGUUgggCgGGCUugaagauuucUGGUCa -3' miRNA: 3'- gUUUGgGCUGUCAGa--GgCCGA----------ACCAG- -5' |
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22083 | 3' | -54.6 | NC_004927.1 | + | 71828 | 0.66 | 0.838124 |
Target: 5'- aAGGCCCGcguCGGuaUCUCCGGUaucacgaGGUCg -3' miRNA: 3'- gUUUGGGCu--GUC--AGAGGCCGaa-----CCAG- -5' |
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22083 | 3' | -54.6 | NC_004927.1 | + | 74930 | 0.68 | 0.700277 |
Target: 5'- aAGAUgCGGCAGUaguacCUCCGGgUaaUGGUCa -3' miRNA: 3'- gUUUGgGCUGUCA-----GAGGCCgA--ACCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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