Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22086 | 3' | -58.8 | NC_004927.1 | + | 58526 | 0.66 | 0.610495 |
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Target: 5'- -aCCUCGAUGCUCgcuaugGCCGGugcuGGuGcGGCg -3' miRNA: 3'- uaGGAGCUACGAGag----UGGCU----CC-C-CCG- -5' |
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| 22086 | 3' | -58.8 | NC_004927.1 | + | 32579 | 0.66 | 0.610495 |
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Target: 5'- gGUCgCUCGGgugGCUCaccgCAUCGAGGucGGCg -3' miRNA: 3'- -UAG-GAGCUa--CGAGa---GUGGCUCCc-CCG- -5' |
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| 22086 | 3' | -58.8 | NC_004927.1 | + | 73522 | 0.67 | 0.547771 |
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Target: 5'- uAUUCUUGggGCcuUCcgCAUCGAGGGGGa -3' miRNA: 3'- -UAGGAGCuaCG--AGa-GUGGCUCCCCCg -5' |
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| 22086 | 3' | -58.8 | NC_004927.1 | + | 61919 | 0.67 | 0.547771 |
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Target: 5'- --aCUCGgcGCUCUCcgucacuUCGAguGGGGGCg -3' miRNA: 3'- uagGAGCuaCGAGAGu------GGCU--CCCCCG- -5' |
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| 22086 | 3' | -58.8 | NC_004927.1 | + | 2032 | 0.67 | 0.527277 |
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Target: 5'- uGUCaCUUaGUGCUaCUUuCCGAGGGGGa -3' miRNA: 3'- -UAG-GAGcUACGA-GAGuGGCUCCCCCg -5' |
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| 22086 | 3' | -58.8 | NC_004927.1 | + | 47567 | 0.68 | 0.507077 |
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Target: 5'- --aCUCGGaggGUaacaUCAUCGAGGGGGCu -3' miRNA: 3'- uagGAGCUa--CGag--AGUGGCUCCCCCG- -5' |
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| 22086 | 3' | -58.8 | NC_004927.1 | + | 61753 | 1.08 | 0.000736 |
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Target: 5'- cAUCCUCGAUGCUCUCACCGAGGGGGCc -3' miRNA: 3'- -UAGGAGCUACGAGAGUGGCUCCCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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