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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22086 | 5' | -55.8 | NC_004927.1 | + | 14611 | 0.65 | 0.832062 |
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Target: 5'- aCU-CGUGGUCGUGGgagcggUcuggagcguuccacGGCgCUCGUGGg -3' miRNA: 3'- -GAuGCGCCAGCGCCa-----A--------------CUG-GAGCACC- -5' |
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| 22086 | 5' | -55.8 | NC_004927.1 | + | 22188 | 0.66 | 0.826001 |
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Target: 5'- -aACGgGGUCGCcGUUcuCgUCGUGGg -3' miRNA: 3'- gaUGCgCCAGCGcCAAcuGgAGCACC- -5' |
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| 22086 | 5' | -55.8 | NC_004927.1 | + | 6618 | 0.67 | 0.750988 |
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Target: 5'- aCUACGUGuUUGUGGUcacaGACCUCGUucuGGu -3' miRNA: 3'- -GAUGCGCcAGCGCCAa---CUGGAGCA---CC- -5' |
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| 22086 | 5' | -55.8 | NC_004927.1 | + | 4554 | 0.68 | 0.720754 |
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Target: 5'- aUACGcCGGUCGuCGGUUGGugUCGUu- -3' miRNA: 3'- gAUGC-GCCAGC-GCCAACUggAGCAcc -5' |
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| 22086 | 5' | -55.8 | NC_004927.1 | + | 69314 | 0.69 | 0.659425 |
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Target: 5'- aCUGC-UGGUCGCcauuaucccgaguccGUUGACCUCGaUGGg -3' miRNA: 3'- -GAUGcGCCAGCGc--------------CAACUGGAGC-ACC- -5' |
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| 22086 | 5' | -55.8 | NC_004927.1 | + | 46882 | 0.71 | 0.502718 |
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Target: 5'- -gGCGCGGUCGUGGagGAgaaCCU-GUGGc -3' miRNA: 3'- gaUGCGCCAGCGCCaaCU---GGAgCACC- -5' |
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| 22086 | 5' | -55.8 | NC_004927.1 | + | 52962 | 0.73 | 0.417076 |
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Target: 5'- aUGCGguUGGUCGCcaagcGUUGACCUCGaUGGa -3' miRNA: 3'- gAUGC--GCCAGCGc----CAACUGGAGC-ACC- -5' |
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| 22086 | 5' | -55.8 | NC_004927.1 | + | 61718 | 1.11 | 0.001309 |
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Target: 5'- uCUACGCGGUCGCGGUUGACCUCGUGGa -3' miRNA: 3'- -GAUGCGCCAGCGCCAACUGGAGCACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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