miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22087 5' -55.3 NC_004927.1 + 58566 0.66 0.814214
Target:  5'- gGCCCugAUUGCGUUCGCuguUCAGgcGUCc -3'
miRNA:   3'- gUGGG--UGAUGCGAGCGc--AGUCauCGG- -5'
22087 5' -55.3 NC_004927.1 + 40421 0.66 0.782176
Target:  5'- cCACCCACUACaaGCUCGaaacCGUCuuccgcuGGUuauggaaauacgagGGCCa -3'
miRNA:   3'- -GUGGGUGAUG--CGAGC----GCAG-------UCA--------------UCGG- -5'
22087 5' -55.3 NC_004927.1 + 27788 0.67 0.725865
Target:  5'- uCACCgCcCUGCGCUCGgGcCAGUuugaacagcGCCa -3'
miRNA:   3'- -GUGG-GuGAUGCGAGCgCaGUCAu--------CGG- -5'
22087 5' -55.3 NC_004927.1 + 14582 0.68 0.673172
Target:  5'- -uUCCACgGCGCUCGUggGUCGccAGCCg -3'
miRNA:   3'- guGGGUGaUGCGAGCG--CAGUcaUCGG- -5'
22087 5' -55.3 NC_004927.1 + 65184 0.69 0.641029
Target:  5'- cCACgC-CUACGgUCGCGUCGGUcguGUCu -3'
miRNA:   3'- -GUGgGuGAUGCgAGCGCAGUCAu--CGG- -5'
22087 5' -55.3 NC_004927.1 + 37409 0.71 0.52433
Target:  5'- uCGCCCGCguuaUGCGCUCGCG-CG--AGCUa -3'
miRNA:   3'- -GUGGGUG----AUGCGAGCGCaGUcaUCGG- -5'
22087 5' -55.3 NC_004927.1 + 63900 1.13 0.000848
Target:  5'- aCACCCACUACGCUCGCGUCAGUAGCCg -3'
miRNA:   3'- -GUGGGUGAUGCGAGCGCAGUCAUCGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.