Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22088 | 5' | -56.1 | NC_004927.1 | + | 29339 | 0.66 | 0.771516 |
Target: 5'- --gUCUCUCCGuCGugGUGGucgGGGCu- -3' miRNA: 3'- auuGGAGAGGCuGCugCGCU---UCCGca -5' |
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22088 | 5' | -56.1 | NC_004927.1 | + | 29036 | 0.66 | 0.761621 |
Target: 5'- cGGCCUUgCgGAUGAUGUGucGGCGg -3' miRNA: 3'- aUUGGAGaGgCUGCUGCGCuuCCGCa -5' |
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22088 | 5' | -56.1 | NC_004927.1 | + | 61040 | 0.66 | 0.761621 |
Target: 5'- -cGCUgg-CCGACGAgaGUGAAGGCGc -3' miRNA: 3'- auUGGagaGGCUGCUg-CGCUUCCGCa -5' |
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22088 | 5' | -56.1 | NC_004927.1 | + | 61916 | 0.66 | 0.741461 |
Target: 5'- cGGCgCUCUCCGucacuuCGA-GUGggGGCGc -3' miRNA: 3'- aUUG-GAGAGGCu-----GCUgCGCuuCCGCa -5' |
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22088 | 5' | -56.1 | NC_004927.1 | + | 61442 | 0.66 | 0.741461 |
Target: 5'- cGACCg--CCGACuACGUGAcGGCGa -3' miRNA: 3'- aUUGGagaGGCUGcUGCGCUuCCGCa -5' |
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22088 | 5' | -56.1 | NC_004927.1 | + | 53577 | 0.66 | 0.731219 |
Target: 5'- cAACCUCccggccaacUCCGGCGAgcugguCGUGAccggGGGCGa -3' miRNA: 3'- aUUGGAG---------AGGCUGCU------GCGCU----UCCGCa -5' |
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22088 | 5' | -56.1 | NC_004927.1 | + | 28817 | 0.68 | 0.614622 |
Target: 5'- -cGCCUCgaugCCGggaaccguuGCGGCGUGAagaaGGGCGUc -3' miRNA: 3'- auUGGAGa---GGC---------UGCUGCGCU----UCCGCA- -5' |
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22088 | 5' | -56.1 | NC_004927.1 | + | 10399 | 0.68 | 0.603922 |
Target: 5'- aUGACCaacUC-CCGACGACGCcccacgguGGGGCGUu -3' miRNA: 3'- -AUUGG---AGaGGCUGCUGCGc-------UUCCGCA- -5' |
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22088 | 5' | -56.1 | NC_004927.1 | + | 9068 | 0.69 | 0.593245 |
Target: 5'- aGAUUUCUUCGACgGACGggauggauuCGAGGGCGUc -3' miRNA: 3'- aUUGGAGAGGCUG-CUGC---------GCUUCCGCA- -5' |
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22088 | 5' | -56.1 | NC_004927.1 | + | 53934 | 0.69 | 0.561433 |
Target: 5'- cGACC-CUCCGAuugaauacgucaCGACG-GAAGGCGc -3' miRNA: 3'- aUUGGaGAGGCU------------GCUGCgCUUCCGCa -5' |
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22088 | 5' | -56.1 | NC_004927.1 | + | 46905 | 0.72 | 0.431257 |
Target: 5'- gUGACgC-CUuuGACGACgGCGAGGGCGc -3' miRNA: 3'- -AUUG-GaGAggCUGCUG-CGCUUCCGCa -5' |
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22088 | 5' | -56.1 | NC_004927.1 | + | 66511 | 0.72 | 0.394823 |
Target: 5'- -uGCUUCUCUGaACGACGCuGAGGUGg -3' miRNA: 3'- auUGGAGAGGC-UGCUGCGcUUCCGCa -5' |
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22088 | 5' | -56.1 | NC_004927.1 | + | 64589 | 1.06 | 0.00192 |
Target: 5'- aUAACCUCUCCGACGACGCGAAGGCGUa -3' miRNA: 3'- -AUUGGAGAGGCUGCUGCGCUUCCGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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