Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22089 | 5' | -56 | NC_004927.1 | + | 45917 | 0.66 | 0.769153 |
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Target: 5'- -cAGGGCGaCGACCugCUCGUGgaguuugacgacuacUUCCUu -3' miRNA: 3'- gcUUUCGC-GCUGGugGGGCAC---------------AAGGA- -5' |
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| 22089 | 5' | -56 | NC_004927.1 | + | 35429 | 0.67 | 0.71454 |
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Target: 5'- aCGAGAG-GCGACCACUCCuuacacUCCUc -3' miRNA: 3'- -GCUUUCgCGCUGGUGGGGcaca--AGGA- -5' |
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| 22089 | 5' | -56 | NC_004927.1 | + | 35195 | 0.68 | 0.640664 |
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Target: 5'- uCGAGGGCGuCGGCU--CCCGUGaagUCCUc -3' miRNA: 3'- -GCUUUCGC-GCUGGugGGGCACa--AGGA- -5' |
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| 22089 | 5' | -56 | NC_004927.1 | + | 53692 | 0.7 | 0.566383 |
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Target: 5'- cCGAggguucGAGCGCGACCGgCCCG-GcUCUUg -3' miRNA: 3'- -GCU------UUCGCGCUGGUgGGGCaCaAGGA- -5' |
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| 22089 | 5' | -56 | NC_004927.1 | + | 43429 | 0.71 | 0.494491 |
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Target: 5'- uGAAcAG-GCGACCGuCgCCGUGUUCCUc -3' miRNA: 3'- gCUU-UCgCGCUGGU-GgGGCACAAGGA- -5' |
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| 22089 | 5' | -56 | NC_004927.1 | + | 683 | 0.71 | 0.464947 |
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Target: 5'- aGggGGC-CGACCGCCCCG-GUUa-- -3' miRNA: 3'- gCuuUCGcGCUGGUGGGGCaCAAgga -5' |
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| 22089 | 5' | -56 | NC_004927.1 | + | 66010 | 1.08 | 0.001597 |
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Target: 5'- uCGAAAGCGCGACCACCCCGUGUUCCUa -3' miRNA: 3'- -GCUUUCGCGCUGGUGGGGCACAAGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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