Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2209 | 3' | -55.6 | NC_001403.1 | + | 1742 | 0.66 | 0.114502 |
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Target: 5'- aGC-UGGCagcgGGGcCGCuGGCCAAGCUg -3' miRNA: 3'- -CGaACCGga--CUC-GUGuCUGGUUCGGg -5' |
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| 2209 | 3' | -55.6 | NC_001403.1 | + | 3751 | 0.67 | 0.089967 |
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Target: 5'- aGgUUGGCgUaGGGCACGGcgcCCAGGCUg -3' miRNA: 3'- -CgAACCGgA-CUCGUGUCu--GGUUCGGg -5' |
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| 2209 | 3' | -55.6 | NC_001403.1 | + | 1021 | 0.67 | 0.086898 |
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Target: 5'- ---cGGCCcagGGGC-CAGACCcgugcGCCCu -3' miRNA: 3'- cgaaCCGGa--CUCGuGUCUGGuu---CGGG- -5' |
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| 2209 | 3' | -55.6 | NC_001403.1 | + | 1667 | 0.73 | 0.026056 |
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Target: 5'- uGCUgUGGUCgguGGCACGGAgaugucCCAGGCCCu -3' miRNA: 3'- -CGA-ACCGGac-UCGUGUCU------GGUUCGGG- -5' |
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| 2209 | 3' | -55.6 | NC_001403.1 | + | 1928 | 0.79 | 0.008212 |
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Target: 5'- cGCUUGGCCUGGGUGCuGucacgcACCAGGCUg -3' miRNA: 3'- -CGAACCGGACUCGUGuC------UGGUUCGGg -5' |
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| 2209 | 3' | -55.6 | NC_001403.1 | + | 2568 | 0.84 | 0.002974 |
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Target: 5'- gGCUUGGUCUGuGCuCAGGCCAAGCUg -3' miRNA: 3'- -CGAACCGGACuCGuGUCUGGUUCGGg -5' |
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| 2209 | 3' | -55.6 | NC_001403.1 | + | 2597 | 1.14 | 0.000007 |
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Target: 5'- aGCUUGGCCUGAGCACAGACCAAGCCCu -3' miRNA: 3'- -CGAACCGGACUCGUGUCUGGUUCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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