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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22092 | 3' | -55.6 | NC_004927.1 | + | 24539 | 0.66 | 0.834716 |
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Target: 5'- cGGUGUUCAGCGCcgggauguGGCGUaUUCGgauGCCg -3' miRNA: 3'- cCCACAAGUUGUGc-------CCGCG-AGGC---UGG- -5' |
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| 22092 | 3' | -55.6 | NC_004927.1 | + | 31894 | 0.66 | 0.817199 |
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Target: 5'- gGGGcUGUUaCAuccGCACGGuGCGUUCUG-CUg -3' miRNA: 3'- -CCC-ACAA-GU---UGUGCC-CGCGAGGCuGG- -5' |
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| 22092 | 3' | -55.6 | NC_004927.1 | + | 33472 | 0.66 | 0.808165 |
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Target: 5'- -cGUGUUCcuuGAgGCGGaGUGCUUCGuCCa -3' miRNA: 3'- ccCACAAG---UUgUGCC-CGCGAGGCuGG- -5' |
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| 22092 | 3' | -55.6 | NC_004927.1 | + | 49281 | 0.67 | 0.780077 |
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Target: 5'- aGGGgucguggGUUCuucuaaGCGaGGaCGCUCcCGACCa -3' miRNA: 3'- -CCCa------CAAGuug---UGC-CC-GCGAG-GCUGG- -5' |
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| 22092 | 3' | -55.6 | NC_004927.1 | + | 65534 | 0.67 | 0.770417 |
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Target: 5'- uGG-GUUCAGCgaaagAUGGGCGgUUuuGACCu -3' miRNA: 3'- cCCaCAAGUUG-----UGCCCGC-GAggCUGG- -5' |
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| 22092 | 3' | -55.6 | NC_004927.1 | + | 53958 | 0.77 | 0.254281 |
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Target: 5'- cGGGgacggagGUUCAACACGGaGCGaccCUCCGAUUg -3' miRNA: 3'- -CCCa------CAAGUUGUGCC-CGC---GAGGCUGG- -5' |
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| 22092 | 3' | -55.6 | NC_004927.1 | + | 61475 | 0.78 | 0.202621 |
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Target: 5'- cGGUGggUAGCACGGGCGaCUCgguggacuacgCGACCg -3' miRNA: 3'- cCCACaaGUUGUGCCCGC-GAG-----------GCUGG- -5' |
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| 22092 | 3' | -55.6 | NC_004927.1 | + | 69005 | 1.13 | 0.000849 |
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Target: 5'- cGGGUGUUCAACACGGGCGCUCCGACCc -3' miRNA: 3'- -CCCACAAGUUGUGCCCGCGAGGCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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