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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22092 | 5' | -55.6 | NC_004927.1 | + | 33221 | 0.66 | 0.768166 |
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Target: 5'- -aGGccguUGAA-CGCCgggaaCACGUAgGCCCCg -3' miRNA: 3'- caCC----ACUUcGCGGa----GUGCAUgUGGGG- -5' |
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| 22092 | 5' | -55.6 | NC_004927.1 | + | 63756 | 0.66 | 0.758212 |
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Target: 5'- aUGaGUGGAuGCGCgacguggauaCUCugGaagACACCCCg -3' miRNA: 3'- cAC-CACUU-CGCG----------GAGugCa--UGUGGGG- -5' |
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| 22092 | 5' | -55.6 | NC_004927.1 | + | 18847 | 0.67 | 0.737945 |
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Target: 5'- -gGGcUGAAGUaCCUCAccCGUugcguCACCCCa -3' miRNA: 3'- caCC-ACUUCGcGGAGU--GCAu----GUGGGG- -5' |
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| 22092 | 5' | -55.6 | NC_004927.1 | + | 14547 | 0.67 | 0.737945 |
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Target: 5'- -cGGUGGguAGUuaCUCACGcuucCACCCCc -3' miRNA: 3'- caCCACU--UCGcgGAGUGCau--GUGGGG- -5' |
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| 22092 | 5' | -55.6 | NC_004927.1 | + | 32853 | 0.67 | 0.712053 |
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Target: 5'- uUGGUGuccuuccAAGCGUCcaucaggucguagCAgGUACGCCCCu -3' miRNA: 3'- cACCAC-------UUCGCGGa------------GUgCAUGUGGGG- -5' |
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| 22092 | 5' | -55.6 | NC_004927.1 | + | 10374 | 0.68 | 0.685693 |
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Target: 5'- -cGGUGggGCGUUUCGcCGUuUACUCUc -3' miRNA: 3'- caCCACuuCGCGGAGU-GCAuGUGGGG- -5' |
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| 22092 | 5' | -55.6 | NC_004927.1 | + | 68970 | 1.12 | 0.000808 |
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Target: 5'- aGUGGUGAAGCGCCUCACGUACACCCCg -3' miRNA: 3'- -CACCACUUCGCGGAGUGCAUGUGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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