Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22093 | 3' | -51.1 | NC_004927.1 | + | 24702 | 0.67 | 0.924223 |
Target: 5'- gGCGuuccguacuucGAGGGCGACcucccggCGUccuGCCGCCGa -3' miRNA: 3'- -CGC-----------UUCUUGCUGuua----GCAu--CGGCGGC- -5' |
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22093 | 3' | -51.1 | NC_004927.1 | + | 59129 | 0.66 | 0.967087 |
Target: 5'- uGCGAAGAcccucgauggguuagGCGACAAUC-UGGuuGaCGa -3' miRNA: 3'- -CGCUUCU---------------UGCUGUUAGcAUCggCgGC- -5' |
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22093 | 3' | -51.1 | NC_004927.1 | + | 40179 | 0.66 | 0.962161 |
Target: 5'- gGCGAcGAGCGAgacuaccuccgaCGAgccgCGUucgAGCCGCUGu -3' miRNA: 3'- -CGCUuCUUGCU------------GUUa---GCA---UCGGCGGC- -5' |
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22093 | 3' | -51.1 | NC_004927.1 | + | 14596 | 0.66 | 0.961791 |
Target: 5'- aGCGGucuGGAgcguuccACGGCGcUCGUGGgUCGCCa -3' miRNA: 3'- -CGCU---UCU-------UGCUGUuAGCAUC-GGCGGc -5' |
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22093 | 3' | -51.1 | NC_004927.1 | + | 14514 | 0.66 | 0.957947 |
Target: 5'- cGgGAAGugGACGACGGccCGUAGCUcaggauaGCCGa -3' miRNA: 3'- -CgCUUC--UUGCUGUUa-GCAUCGG-------CGGC- -5' |
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22093 | 3' | -51.1 | NC_004927.1 | + | 10911 | 0.66 | 0.95427 |
Target: 5'- uCGggGAGgGAgcaguccuuaCAGUUGUcGCCGCUGg -3' miRNA: 3'- cGCuuCUUgCU----------GUUAGCAuCGGCGGC- -5' |
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22093 | 3' | -51.1 | NC_004927.1 | + | 3012 | 0.66 | 0.949935 |
Target: 5'- cCGggGAGuCGAacccCGgAGCCGCCGc -3' miRNA: 3'- cGCuuCUU-GCUguuaGCaUCGGCGGC- -5' |
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22093 | 3' | -51.1 | NC_004927.1 | + | 20469 | 0.66 | 0.949935 |
Target: 5'- cCGAAGGguuCGAgAGUCGcuucgaGGUCGCCGg -3' miRNA: 3'- cGCUUCUu--GCUgUUAGCa-----UCGGCGGC- -5' |
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22093 | 3' | -51.1 | NC_004927.1 | + | 71964 | 0.67 | 0.940465 |
Target: 5'- -aGAGGAcGCGAUGGggcgCGgAGCCGCUGa -3' miRNA: 3'- cgCUUCU-UGCUGUUa---GCaUCGGCGGC- -5' |
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22093 | 3' | -51.1 | NC_004927.1 | + | 47173 | 0.67 | 0.940465 |
Target: 5'- gGUGAagcAGGGCGGCAagcGUCGUGG-CGCUc -3' miRNA: 3'- -CGCU---UCUUGCUGU---UAGCAUCgGCGGc -5' |
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22093 | 3' | -51.1 | NC_004927.1 | + | 71101 | 0.67 | 0.935325 |
Target: 5'- gGCGAgcGGAugGACu-UC---GCCGCCGa -3' miRNA: 3'- -CGCU--UCUugCUGuuAGcauCGGCGGC- -5' |
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22093 | 3' | -51.1 | NC_004927.1 | + | 69524 | 1.11 | 0.003351 |
Target: 5'- uGCGAAGAACGACAAUCGUAGCCGCCGu -3' miRNA: 3'- -CGCUUCUUGCUGUUAGCAUCGGCGGC- -5' |
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22093 | 3' | -51.1 | NC_004927.1 | + | 2324 | 0.67 | 0.924223 |
Target: 5'- gGCGAggaAGAACGcucuccCAAUUG-AGCCGUCGc -3' miRNA: 3'- -CGCU---UCUUGCu-----GUUAGCaUCGGCGGC- -5' |
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22093 | 3' | -51.1 | NC_004927.1 | + | 63913 | 0.68 | 0.916419 |
Target: 5'- gGUGAGGugauUGACAcccacuacgcucgcGUCaGUAGCCGCCc -3' miRNA: 3'- -CGCUUCuu--GCUGU--------------UAG-CAUCGGCGGc -5' |
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22093 | 3' | -51.1 | NC_004927.1 | + | 37834 | 0.68 | 0.905519 |
Target: 5'- uCGGAGGGCuggucggcuuucGACAcaucgaGUCGU-GCCGCCGu -3' miRNA: 3'- cGCUUCUUG------------CUGU------UAGCAuCGGCGGC- -5' |
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22093 | 3' | -51.1 | NC_004927.1 | + | 73358 | 0.68 | 0.904853 |
Target: 5'- uGCGAA-AACuGCGAUUGUGGCCuuucugaGCCGg -3' miRNA: 3'- -CGCUUcUUGcUGUUAGCAUCGG-------CGGC- -5' |
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22093 | 3' | -51.1 | NC_004927.1 | + | 50245 | 0.68 | 0.898743 |
Target: 5'- cUGGAGGugGCGGCGGUgGUGGUggaGCCGg -3' miRNA: 3'- cGCUUCU--UGCUGUUAgCAUCGg--CGGC- -5' |
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22093 | 3' | -51.1 | NC_004927.1 | + | 26629 | 0.69 | 0.884399 |
Target: 5'- cGCGucguGAGCGACGggaaugaacucGUCGUAGuaGCCc -3' miRNA: 3'- -CGCuu--CUUGCUGU-----------UAGCAUCggCGGc -5' |
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22093 | 3' | -51.1 | NC_004927.1 | + | 14645 | 0.69 | 0.852686 |
Target: 5'- uCGAGGAugGgAUAGUCGgGGUCGCaCGg -3' miRNA: 3'- cGCUUCUugC-UGUUAGCaUCGGCG-GC- -5' |
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22093 | 3' | -51.1 | NC_004927.1 | + | 49201 | 0.72 | 0.716905 |
Target: 5'- cGCGAGGAugGACAAaaa-GGCCGCg- -3' miRNA: 3'- -CGCUUCUugCUGUUagcaUCGGCGgc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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