Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22093 | 5' | -53.7 | NC_004927.1 | + | 71738 | 0.7 | 0.681954 |
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Target: 5'- cGGCGGCGG---UGGCUgaGCGAauCUCUCa -3' miRNA: 3'- -CCGCCGCUaauGCUGA--CGCU--GAGAGc -5' |
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| 22093 | 5' | -53.7 | NC_004927.1 | + | 69480 | 1.1 | 0.002076 |
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Target: 5'- gGGCGGCGAUUACGACUGCGACUCUCGc -3' miRNA: 3'- -CCGCCGCUAAUGCUGACGCUGAGAGC- -5' |
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| 22093 | 5' | -53.7 | NC_004927.1 | + | 67797 | 0.67 | 0.830652 |
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Target: 5'- cGGCGGCGugacGCaGACUGCucC-CUCGg -3' miRNA: 3'- -CCGCCGCuaa-UG-CUGACGcuGaGAGC- -5' |
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| 22093 | 5' | -53.7 | NC_004927.1 | + | 64774 | 0.66 | 0.893915 |
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Target: 5'- cGGUGGCGAUU-CGA--GCG-UUCUCa -3' miRNA: 3'- -CCGCCGCUAAuGCUgaCGCuGAGAGc -5' |
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| 22093 | 5' | -53.7 | NC_004927.1 | + | 60783 | 0.68 | 0.784412 |
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Target: 5'- cGGUGucccgaguCGAUgcgACGACUGCGGCUgUUGa -3' miRNA: 3'- -CCGCc-------GCUAa--UGCUGACGCUGAgAGC- -5' |
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| 22093 | 5' | -53.7 | NC_004927.1 | + | 59371 | 0.76 | 0.379184 |
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Target: 5'- cGGCGGCGGUaAUGGuCgGCG-CUCUCGg -3' miRNA: 3'- -CCGCCGCUAaUGCU-GaCGCuGAGAGC- -5' |
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| 22093 | 5' | -53.7 | NC_004927.1 | + | 50751 | 0.7 | 0.713646 |
|
Target: 5'- uGGCGGUGAUggugGCGAUgcugGCGGUUCUa- -3' miRNA: 3'- -CCGCCGCUAa---UGCUGa---CGCUGAGAgc -5' |
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| 22093 | 5' | -53.7 | NC_004927.1 | + | 34514 | 0.74 | 0.462131 |
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Target: 5'- cGCGGCGAgucCGGuCUGCGACUgUCc -3' miRNA: 3'- cCGCCGCUaauGCU-GACGCUGAgAGc -5' |
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| 22093 | 5' | -53.7 | NC_004927.1 | + | 10646 | 0.69 | 0.734408 |
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Target: 5'- cGGuCGGCccgucGAUUACGACguucgUGUGACcCUCGa -3' miRNA: 3'- -CC-GCCG-----CUAAUGCUG-----ACGCUGaGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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