Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22094 | 3' | -55.9 | NC_004927.1 | + | 53467 | 0.66 | 0.729714 |
Target: 5'- uGAacGGCGuuuCCGUCUCGGgaacGCUUGCUg -3' miRNA: 3'- gCU--UCGUu--GGCGGAGCUa---CGGACGAa -5' |
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22094 | 3' | -55.9 | NC_004927.1 | + | 15065 | 0.68 | 0.621915 |
Target: 5'- uCGGAGCGAagcCCGCCgCGAcUGCCggGCa- -3' miRNA: 3'- -GCUUCGUU---GGCGGaGCU-ACGGa-CGaa -5' |
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22094 | 3' | -55.9 | NC_004927.1 | + | 6394 | 0.69 | 0.54632 |
Target: 5'- cCGGAGCuuugAGCCGCCaCGAgagGCgUGCUUc -3' miRNA: 3'- -GCUUCG----UUGGCGGaGCUa--CGgACGAA- -5' |
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22094 | 3' | -55.9 | NC_004927.1 | + | 70186 | 1.05 | 0.00204 |
Target: 5'- gCGAAGCAACCGCCUCGAUGCCUGCUUu -3' miRNA: 3'- -GCUUCGUUGGCGGAGCUACGGACGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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