miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22095 3' -60 NC_004927.1 + 24000 0.66 0.570775
Target:  5'- aACCGGGUGCuuGCccuccgUGGAGCguccggccccaCGUCGGGa -3'
miRNA:   3'- -UGGUUCAUGcgCG------ACCUCG-----------GCGGCCC- -5'
22095 3' -60 NC_004927.1 + 47518 0.69 0.373137
Target:  5'- cGCUGAGaACGuCGCcGGAGCCGaggaagaCGGGg -3'
miRNA:   3'- -UGGUUCaUGC-GCGaCCUCGGCg------GCCC- -5'
22095 3' -60 NC_004927.1 + 46109 0.69 0.381478
Target:  5'- gACCGAGUACGaCGagUGGuucGCCGCucgcaCGGGg -3'
miRNA:   3'- -UGGUUCAUGC-GCg-ACCu--CGGCG-----GCCC- -5'
22095 3' -60 NC_004927.1 + 38720 0.7 0.35683
Target:  5'- aACCGAGUcggccCGCGCcguggGGAGCCGaguaguuguauUCGGGg -3'
miRNA:   3'- -UGGUUCAu----GCGCGa----CCUCGGC-----------GGCCC- -5'
22095 3' -60 NC_004927.1 + 21807 0.8 0.077704
Target:  5'- cACCGAGcgaGC-CGCUGGAGCCGCUGGa -3'
miRNA:   3'- -UGGUUCa--UGcGCGACCUCGGCGGCCc -5'
22095 3' -60 NC_004927.1 + 70662 1.09 0.000573
Target:  5'- aACCAAGUACGCGCUGGAGCCGCCGGGu -3'
miRNA:   3'- -UGGUUCAUGCGCGACCUCGGCGGCCC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.