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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 22095 | 3' | -60 | NC_004927.1 | + | 24000 | 0.66 | 0.570775 |
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Target: 5'- aACCGGGUGCuuGCccuccgUGGAGCguccggccccaCGUCGGGa -3' miRNA: 3'- -UGGUUCAUGcgCG------ACCUCG-----------GCGGCCC- -5' |
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| 22095 | 3' | -60 | NC_004927.1 | + | 47518 | 0.69 | 0.373137 |
|
Target: 5'- cGCUGAGaACGuCGCcGGAGCCGaggaagaCGGGg -3' miRNA: 3'- -UGGUUCaUGC-GCGaCCUCGGCg------GCCC- -5' |
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| 22095 | 3' | -60 | NC_004927.1 | + | 46109 | 0.69 | 0.381478 |
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Target: 5'- gACCGAGUACGaCGagUGGuucGCCGCucgcaCGGGg -3' miRNA: 3'- -UGGUUCAUGC-GCg-ACCu--CGGCG-----GCCC- -5' |
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| 22095 | 3' | -60 | NC_004927.1 | + | 38720 | 0.7 | 0.35683 |
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Target: 5'- aACCGAGUcggccCGCGCcguggGGAGCCGaguaguuguauUCGGGg -3' miRNA: 3'- -UGGUUCAu----GCGCGa----CCUCGGC-----------GGCCC- -5' |
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| 22095 | 3' | -60 | NC_004927.1 | + | 21807 | 0.8 | 0.077704 |
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Target: 5'- cACCGAGcgaGC-CGCUGGAGCCGCUGGa -3' miRNA: 3'- -UGGUUCa--UGcGCGACCUCGGCGGCCc -5' |
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| 22095 | 3' | -60 | NC_004927.1 | + | 70662 | 1.09 | 0.000573 |
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Target: 5'- aACCAAGUACGCGCUGGAGCCGCCGGGu -3' miRNA: 3'- -UGGUUCAUGCGCGACCUCGGCGGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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