Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22095 | 5' | -55.3 | NC_004927.1 | + | 33833 | 0.66 | 0.837723 |
Target: 5'- aUCCCGuacuCGGCGGCGAgGuCAACGu-- -3' miRNA: 3'- cGGGGCu---GCUGCUGCUgC-GUUGCuaa -5' |
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22095 | 5' | -55.3 | NC_004927.1 | + | 65914 | 0.66 | 0.801964 |
Target: 5'- uCCCCGACcguguACGAC-ACGCAcguuGCGAUUu -3' miRNA: 3'- cGGGGCUGc----UGCUGcUGCGU----UGCUAA- -5' |
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22095 | 5' | -55.3 | NC_004927.1 | + | 54750 | 0.66 | 0.801964 |
Target: 5'- aCCCCGGCG-CGGgGACaGCGACcGUg -3' miRNA: 3'- cGGGGCUGCuGCUgCUG-CGUUGcUAa -5' |
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22095 | 5' | -55.3 | NC_004927.1 | + | 60780 | 0.66 | 0.792583 |
Target: 5'- uGUCCCGAguCGauGCGACGACuGCGGCuGUUg -3' miRNA: 3'- -CGGGGCU--GC--UGCUGCUG-CGUUGcUAA- -5' |
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22095 | 5' | -55.3 | NC_004927.1 | + | 65506 | 0.66 | 0.792583 |
Target: 5'- aCCUCGGCGGCaACGAaaGCGACGGc- -3' miRNA: 3'- cGGGGCUGCUGcUGCUg-CGUUGCUaa -5' |
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22095 | 5' | -55.3 | NC_004927.1 | + | 53203 | 0.67 | 0.763538 |
Target: 5'- uUCCCGGCGuCuACGACGUuACGAg- -3' miRNA: 3'- cGGGGCUGCuGcUGCUGCGuUGCUaa -5' |
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22095 | 5' | -55.3 | NC_004927.1 | + | 55140 | 0.67 | 0.753589 |
Target: 5'- cGUCaaaGCGAcCGGCGACGUGACGAUUg -3' miRNA: 3'- -CGGggcUGCU-GCUGCUGCGUUGCUAA- -5' |
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22095 | 5' | -55.3 | NC_004927.1 | + | 42324 | 0.67 | 0.743524 |
Target: 5'- aGCCCguUGGCGGCGACGGgGUAGgcauCGAg- -3' miRNA: 3'- -CGGG--GCUGCUGCUGCUgCGUU----GCUaa -5' |
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22095 | 5' | -55.3 | NC_004927.1 | + | 67806 | 0.68 | 0.69182 |
Target: 5'- cGCUCCG--GGCGGCGGCGUGACGc-- -3' miRNA: 3'- -CGGGGCugCUGCUGCUGCGUUGCuaa -5' |
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22095 | 5' | -55.3 | NC_004927.1 | + | 67861 | 0.69 | 0.670688 |
Target: 5'- aCCUCGAaGACGAgGACGaGACGGUUg -3' miRNA: 3'- cGGGGCUgCUGCUgCUGCgUUGCUAA- -5' |
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22095 | 5' | -55.3 | NC_004927.1 | + | 45508 | 0.69 | 0.670688 |
Target: 5'- aUCUgGACGACGaggGCGAcCGCGACGAc- -3' miRNA: 3'- cGGGgCUGCUGC---UGCU-GCGUUGCUaa -5' |
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22095 | 5' | -55.3 | NC_004927.1 | + | 46901 | 0.69 | 0.670688 |
Target: 5'- cGCCUuugaCGACGGCGAgGGCGCGGuCGu-- -3' miRNA: 3'- -CGGG----GCUGCUGCUgCUGCGUU-GCuaa -5' |
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22095 | 5' | -55.3 | NC_004927.1 | + | 52846 | 0.69 | 0.660066 |
Target: 5'- aGCUCCGGCGGCGcuccggugaAgGACGCGACc--- -3' miRNA: 3'- -CGGGGCUGCUGC---------UgCUGCGUUGcuaa -5' |
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22095 | 5' | -55.3 | NC_004927.1 | + | 73082 | 0.69 | 0.649419 |
Target: 5'- cUCCCGugGACGGCGAaGCcguCGGUc -3' miRNA: 3'- cGGGGCugCUGCUGCUgCGuu-GCUAa -5' |
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22095 | 5' | -55.3 | NC_004927.1 | + | 73882 | 0.69 | 0.649419 |
Target: 5'- aCCCCGACGAUGAaauauuCGAUGaCGAUGGc- -3' miRNA: 3'- cGGGGCUGCUGCU------GCUGC-GUUGCUaa -5' |
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22095 | 5' | -55.3 | NC_004927.1 | + | 45656 | 0.69 | 0.638757 |
Target: 5'- cUCCCGaACGAggcgacacaCGACGACGUGGCGGa- -3' miRNA: 3'- cGGGGC-UGCU---------GCUGCUGCGUUGCUaa -5' |
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22095 | 5' | -55.3 | NC_004927.1 | + | 63874 | 0.69 | 0.628091 |
Target: 5'- cGCCCCGguuACGACGACucCGCuggUGAUUa -3' miRNA: 3'- -CGGGGC---UGCUGCUGcuGCGuu-GCUAA- -5' |
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22095 | 5' | -55.3 | NC_004927.1 | + | 73237 | 0.69 | 0.616361 |
Target: 5'- aCCCCGcUGACGugGAaaaaaucgaaauuUGCGGCGAUa -3' miRNA: 3'- cGGGGCuGCUGCugCU-------------GCGUUGCUAa -5' |
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22095 | 5' | -55.3 | NC_004927.1 | + | 71112 | 0.7 | 0.606775 |
Target: 5'- cUCCCGACGAgGGCGAgCGgAugGAc- -3' miRNA: 3'- cGGGGCUGCUgCUGCU-GCgUugCUaa -5' |
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22095 | 5' | -55.3 | NC_004927.1 | + | 58610 | 0.7 | 0.596144 |
Target: 5'- cCCUCGAUGGCGAagugGugGCAACuGAUUg -3' miRNA: 3'- cGGGGCUGCUGCUg---CugCGUUG-CUAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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