Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22096 | 5' | -44 | NC_004927.1 | + | 45822 | 0.66 | 0.999957 |
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Target: 5'- gACGCUGAUgcGGAACaaCGAGuuuGAUgGAg -3' miRNA: 3'- -UGCGACUAauUCUUGc-GCUU---UUAgCU- -5' |
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| 22096 | 5' | -44 | NC_004927.1 | + | 52837 | 0.66 | 0.999941 |
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Target: 5'- gGCGCUccGGUgAAGGACGCGAccacuacCGAg -3' miRNA: 3'- -UGCGA--CUAaUUCUUGCGCUuuua---GCU- -5' |
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| 22096 | 5' | -44 | NC_004927.1 | + | 19807 | 0.66 | 0.99992 |
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Target: 5'- gACGgaGGggAAGcGACcgGCGGGAAUCGAa -3' miRNA: 3'- -UGCgaCUaaUUC-UUG--CGCUUUUAGCU- -5' |
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| 22096 | 5' | -44 | NC_004927.1 | + | 49219 | 0.66 | 0.99992 |
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Target: 5'- cACGUaacGAUguucGAGCGCGAGGAUgGAc -3' miRNA: 3'- -UGCGa--CUAauu-CUUGCGCUUUUAgCU- -5' |
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| 22096 | 5' | -44 | NC_004927.1 | + | 13590 | 0.66 | 0.999893 |
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Target: 5'- cCGaCUGAUUGAGccgaGCG-AGGUCGAg -3' miRNA: 3'- uGC-GACUAAUUCuug-CGCuUUUAGCU- -5' |
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| 22096 | 5' | -44 | NC_004927.1 | + | 61170 | 0.67 | 0.999812 |
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Target: 5'- uACGCUGGccucc-GCGCGAAGcUCGGa -3' miRNA: 3'- -UGCGACUaauucuUGCGCUUUuAGCU- -5' |
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| 22096 | 5' | -44 | NC_004927.1 | + | 39804 | 0.68 | 0.999478 |
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Target: 5'- aACGCUGGauggAGGAACGC-AAGA-CGAu -3' miRNA: 3'- -UGCGACUaa--UUCUUGCGcUUUUaGCU- -5' |
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| 22096 | 5' | -44 | NC_004927.1 | + | 46323 | 0.69 | 0.998418 |
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Target: 5'- aGCGCgucGGU--GGGAUGCgGAAAAUCGGa -3' miRNA: 3'- -UGCGa--CUAauUCUUGCG-CUUUUAGCU- -5' |
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| 22096 | 5' | -44 | NC_004927.1 | + | 60637 | 0.7 | 0.995925 |
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Target: 5'- aACGUUGAccggaUUGAGAGCGCGG----CGAa -3' miRNA: 3'- -UGCGACU-----AAUUCUUGCGCUuuuaGCU- -5' |
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| 22096 | 5' | -44 | NC_004927.1 | + | 72489 | 1.11 | 0.018625 |
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Target: 5'- gACGCUGAUUAAGAACGCGAAAAUCGAc -3' miRNA: 3'- -UGCGACUAAUUCUUGCGCUUUUAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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