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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22097 | 3' | -51.2 | NC_004927.1 | + | 46898 | 0.66 | 0.955393 |
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Target: 5'- cUUUGACGACggcgaGGGCGCGGucguGGAGgaGAACc -3' miRNA: 3'- -AGACUGUUG-----UCUGUGCC----UCUCg-CUUG- -5' |
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| 22097 | 3' | -51.2 | NC_004927.1 | + | 20229 | 0.66 | 0.95107 |
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Target: 5'- --cGGgGACGGACuugccACGGAGGuCGAACa -3' miRNA: 3'- agaCUgUUGUCUG-----UGCCUCUcGCUUG- -5' |
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| 22097 | 3' | -51.2 | NC_004927.1 | + | 30615 | 0.67 | 0.925326 |
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Target: 5'- -gUGACGACGGAgACGGucugcuuuAGCGAGa -3' miRNA: 3'- agACUGUUGUCUgUGCCuc------UCGCUUg -5' |
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| 22097 | 3' | -51.2 | NC_004927.1 | + | 75765 | 0.67 | 0.913065 |
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Target: 5'- --aGACgGACGGGCAgGGGGcAGgGGACg -3' miRNA: 3'- agaCUG-UUGUCUGUgCCUC-UCgCUUG- -5' |
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| 22097 | 3' | -51.2 | NC_004927.1 | + | 248 | 0.67 | 0.913065 |
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Target: 5'- --aGACgGACGGGCAgGGGGcAGgGGACg -3' miRNA: 3'- agaCUG-UUGUCUGUgCCUC-UCgCUUG- -5' |
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| 22097 | 3' | -51.2 | NC_004927.1 | + | 442 | 0.69 | 0.826639 |
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Target: 5'- --cGGgGAgAGACGgGGAGAGaCGGACg -3' miRNA: 3'- agaCUgUUgUCUGUgCCUCUC-GCUUG- -5' |
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| 22097 | 3' | -51.2 | NC_004927.1 | + | 68895 | 0.7 | 0.798234 |
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Target: 5'- gUUGugAAC-GACgACGGAGAGCcuGAACu -3' miRNA: 3'- aGACugUUGuCUG-UGCCUCUCG--CUUG- -5' |
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| 22097 | 3' | -51.2 | NC_004927.1 | + | 75145 | 1.1 | 0.00338 |
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Target: 5'- cUCUGACAACAGACACGGAGAGCGAACc -3' miRNA: 3'- -AGACUGUUGUCUGUGCCUCUCGCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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