Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22098 | 3' | -47.5 | NC_004927.1 | + | 50511 | 0.82 | 0.408454 |
Target: 5'- cGGAAACGGCGGAaaUUCGGUGA--AUGGa -3' miRNA: 3'- -CUUUUGCCGCCU--AAGCCAUUcaUGCC- -5' |
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22098 | 3' | -47.5 | NC_004927.1 | + | 62899 | 0.73 | 0.889666 |
Target: 5'- uGAGAGC-GCGGGUUCGGUAcGUAUc- -3' miRNA: 3'- -CUUUUGcCGCCUAAGCCAUuCAUGcc -5' |
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22098 | 3' | -47.5 | NC_004927.1 | + | 50154 | 0.72 | 0.896952 |
Target: 5'- cGGAGAUGGCGGAgaUGGUGGuGUACc- -3' miRNA: 3'- -CUUUUGCCGCCUaaGCCAUU-CAUGcc -5' |
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22098 | 3' | -47.5 | NC_004927.1 | + | 73934 | 0.72 | 0.910689 |
Target: 5'- ---uGCGGCGGAggcuguucUCGGUGGGggagaaGCGGc -3' miRNA: 3'- cuuuUGCCGCCUa-------AGCCAUUCa-----UGCC- -5' |
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22098 | 3' | -47.5 | NC_004927.1 | + | 57632 | 0.72 | 0.923289 |
Target: 5'- cGAAuuACGGCGGugcuggcUCGGguAGUACGGu -3' miRNA: 3'- -CUUu-UGCCGCCua-----AGCCauUCAUGCC- -5' |
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22098 | 3' | -47.5 | NC_004927.1 | + | 58926 | 0.71 | 0.934738 |
Target: 5'- gGAAGGCGcG-GGAUUCGGcacGAGUGCGa -3' miRNA: 3'- -CUUUUGC-CgCCUAAGCCa--UUCAUGCc -5' |
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22098 | 3' | -47.5 | NC_004927.1 | + | 65493 | 0.71 | 0.940032 |
Target: 5'- cGAAagcGACGGCGGGagUGGUGGGaACGa -3' miRNA: 3'- -CUU---UUGCCGCCUaaGCCAUUCaUGCc -5' |
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22098 | 3' | -47.5 | NC_004927.1 | + | 26553 | 0.7 | 0.962294 |
Target: 5'- --uAGCGGCGGA---GGUGGGU-CGGc -3' miRNA: 3'- cuuUUGCCGCCUaagCCAUUCAuGCC- -5' |
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22098 | 3' | -47.5 | NC_004927.1 | + | 45442 | 0.7 | 0.954216 |
Target: 5'- aGAAGGCGGCGGuucagcUCGGUAucGUuaGCGa -3' miRNA: 3'- -CUUUUGCCGCCua----AGCCAUu-CA--UGCc -5' |
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22098 | 3' | -47.5 | NC_004927.1 | + | 50391 | 0.69 | 0.972465 |
Target: 5'- cGGGAGCGGUGGuggUGGUGcugGCGGu -3' miRNA: 3'- -CUUUUGCCGCCuaaGCCAUucaUGCC- -5' |
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22098 | 3' | -47.5 | NC_004927.1 | + | 427 | 0.68 | 0.988299 |
Target: 5'- aGAGACGGaCGGAacCGGaGAG-ACGGa -3' miRNA: 3'- cUUUUGCC-GCCUaaGCCaUUCaUGCC- -5' |
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22098 | 3' | -47.5 | NC_004927.1 | + | 50032 | 0.68 | 0.989806 |
Target: 5'- -uGGACGGUGGggUUGGUGguucaAGUaacucacgaGCGGg -3' miRNA: 3'- cuUUUGCCGCCuaAGCCAU-----UCA---------UGCC- -5' |
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22098 | 3' | -47.5 | NC_004927.1 | + | 50328 | 0.68 | 0.989806 |
Target: 5'- uGGAGGCGGCGGAgcaGGUGgcAGUcuGCu- -3' miRNA: 3'- -CUUUUGCCGCCUaagCCAU--UCA--UGcc -5' |
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22098 | 3' | -47.5 | NC_004927.1 | + | 455 | 0.66 | 0.995214 |
Target: 5'- aGAGACGGaCGGA--CGGgGAGagACGGg -3' miRNA: 3'- cUUUUGCC-GCCUaaGCCaUUCa-UGCC- -5' |
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22098 | 3' | -47.5 | NC_004927.1 | + | 64659 | 0.66 | 0.996581 |
Target: 5'- ---uACGGCGcGAUgCGGgacagGAGUggcGCGGa -3' miRNA: 3'- cuuuUGCCGC-CUAaGCCa----UUCA---UGCC- -5' |
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22098 | 3' | -47.5 | NC_004927.1 | + | 59371 | 0.66 | 0.996812 |
Target: 5'- --cGGCGGCGGuaauggUCGGcGcucucggagugagacAGUACGGu -3' miRNA: 3'- cuuUUGCCGCCua----AGCCaU---------------UCAUGCC- -5' |
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22098 | 3' | -47.5 | NC_004927.1 | + | 46708 | 0.66 | 0.997609 |
Target: 5'- gGGGcgUGGCGGAa-CGGUGAGc-CGGg -3' miRNA: 3'- -CUUuuGCCGCCUaaGCCAUUCauGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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