Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22098 | 5' | -57.2 | NC_004927.1 | + | 926 | 0.67 | 0.686695 |
Target: 5'- uUCCGUCUCcCCGG-UCgGcCCGUa- -3' miRNA: 3'- cAGGCAGAGaGGCCaAGgCaGGCAga -5' |
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22098 | 5' | -57.2 | NC_004927.1 | + | 74759 | 0.67 | 0.676276 |
Target: 5'- aUCU-UCUCUCUGGUUCUGuuuUCUGUCg -3' miRNA: 3'- cAGGcAGAGAGGCCAAGGC---AGGCAGa -5' |
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22098 | 5' | -57.2 | NC_004927.1 | + | 32445 | 0.67 | 0.666865 |
Target: 5'- uGUCCGUaCUCggggaauccuucgccCCGGU--CGUCCGUCUc -3' miRNA: 3'- -CAGGCA-GAGa--------------GGCCAagGCAGGCAGA- -5' |
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22098 | 5' | -57.2 | NC_004927.1 | + | 3472 | 0.68 | 0.592309 |
Target: 5'- -gCCGcUCUCUccgCCGGUUCCcUCCGUg- -3' miRNA: 3'- caGGC-AGAGA---GGCCAAGGcAGGCAga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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