Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22099 | 5' | -62.2 | NC_004927.1 | + | 75798 | 0.78 | 0.09509 |
Target: 5'- gGGGCCGACCGGcccgaGAGACGG-GCaGGGg -3' miRNA: 3'- -CCCGGCUGGCCc----CUCUGCCuUGgCCC- -5' |
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22099 | 5' | -62.2 | NC_004927.1 | + | 281 | 0.78 | 0.09509 |
Target: 5'- gGGGCCGACCGGcccgaGAGACGG-GCaGGGg -3' miRNA: 3'- -CCCGGCUGGCCc----CUCUGCCuUGgCCC- -5' |
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22099 | 5' | -62.2 | NC_004927.1 | + | 441 | 0.72 | 0.217968 |
Target: 5'- gGGGagaGACgGGGaGAGACGGAcggaACCGGa -3' miRNA: 3'- -CCCgg-CUGgCCC-CUCUGCCU----UGGCCc -5' |
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22099 | 5' | -62.2 | NC_004927.1 | + | 35287 | 0.71 | 0.257403 |
Target: 5'- -cGCCGA-UGGGGAGAacGAACCGGGc -3' miRNA: 3'- ccCGGCUgGCCCCUCUgcCUUGGCCC- -5' |
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22099 | 5' | -62.2 | NC_004927.1 | + | 1365 | 0.71 | 0.264722 |
Target: 5'- cGGGCCGuacuucaaacguaacACCGGGaaacacguuCGGGGCCGGGc -3' miRNA: 3'- -CCCGGC---------------UGGCCCcucu-----GCCUUGGCCC- -5' |
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22099 | 5' | -62.2 | NC_004927.1 | + | 59026 | 0.7 | 0.323592 |
Target: 5'- gGGGgCGAuuCCGGuGGAGAC---ACCGGGa -3' miRNA: 3'- -CCCgGCU--GGCC-CCUCUGccuUGGCCC- -5' |
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22099 | 5' | -62.2 | NC_004927.1 | + | 3020 | 0.69 | 0.330867 |
Target: 5'- aGGCCcuuCCGGGGAGucgaacccCGGAGCCGc- -3' miRNA: 3'- cCCGGcu-GGCCCCUCu-------GCCUUGGCcc -5' |
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22099 | 5' | -62.2 | NC_004927.1 | + | 75843 | 0.68 | 0.385118 |
Target: 5'- cGGGCagggGGCgGGGGgagAGACGGcccGCgCGGGg -3' miRNA: 3'- -CCCGg---CUGgCCCC---UCUGCCu--UG-GCCC- -5' |
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22099 | 5' | -62.2 | NC_004927.1 | + | 326 | 0.68 | 0.385118 |
Target: 5'- cGGGCagggGGCgGGGGgagAGACGGcccGCgCGGGg -3' miRNA: 3'- -CCCGg---CUGgCCCC---UCUGCCu--UG-GCCC- -5' |
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22099 | 5' | -62.2 | NC_004927.1 | + | 915 | 0.68 | 0.392508 |
Target: 5'- cGGUCGGCCcguaacaGGGGc--CGGAACCGGa -3' miRNA: 3'- cCCGGCUGG-------CCCCucuGCCUUGGCCc -5' |
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22099 | 5' | -62.2 | NC_004927.1 | + | 31107 | 0.68 | 0.401663 |
Target: 5'- --cCCGACCGGGGA-ACGGAuguucgucgUCGGGg -3' miRNA: 3'- cccGGCUGGCCCCUcUGCCUu--------GGCCC- -5' |
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22099 | 5' | -62.2 | NC_004927.1 | + | 75673 | 0.68 | 0.410103 |
Target: 5'- aGGGUacaagcccgagCGACgGGGGAcAgGGGACgGGGg -3' miRNA: 3'- -CCCG-----------GCUGgCCCCUcUgCCUUGgCCC- -5' |
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22099 | 5' | -62.2 | NC_004927.1 | + | 156 | 0.68 | 0.410103 |
Target: 5'- aGGGUacaagcccgagCGACgGGGGAcAgGGGACgGGGg -3' miRNA: 3'- -CCCG-----------GCUGgCCCCUcUgCCUUGgCCC- -5' |
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22099 | 5' | -62.2 | NC_004927.1 | + | 52453 | 0.67 | 0.453902 |
Target: 5'- aGGGCaaccCGACCGGaGaGGGCGGGACaaaaGGa -3' miRNA: 3'- -CCCG----GCUGGCC-CcUCUGCCUUGg---CCc -5' |
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22099 | 5' | -62.2 | NC_004927.1 | + | 38322 | 0.66 | 0.537557 |
Target: 5'- -aGCCGAaucuucucccaauCCGGGGAGcCGu--CCGGGu -3' miRNA: 3'- ccCGGCU-------------GGCCCCUCuGCcuuGGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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