Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
221 | 3' | -56.5 | AC_000008.1 | + | 17374 | 0.66 | 0.517683 |
Target: 5'- --gUCCAgcguUACGGGUGUGUGCUgGCCc -3' miRNA: 3'- guuGGGU----AUGCUCGCGCGCGAaCGGc -5' |
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221 | 3' | -56.5 | AC_000008.1 | + | 18096 | 0.66 | 0.496275 |
Target: 5'- -cGCgCAUGCGAcgguGCGaCGCGCgccGCCGc -3' miRNA: 3'- guUGgGUAUGCU----CGC-GCGCGaa-CGGC- -5' |
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221 | 3' | -56.5 | AC_000008.1 | + | 8671 | 0.67 | 0.464943 |
Target: 5'- gAGCUgGUGCu-GCGCGCGUaggUUGCUGg -3' miRNA: 3'- gUUGGgUAUGcuCGCGCGCG---AACGGC- -5' |
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221 | 3' | -56.5 | AC_000008.1 | + | 17860 | 0.67 | 0.464943 |
Target: 5'- --uCCCAUgaACGGGCGCGaGCcgUGUCGc -3' miRNA: 3'- guuGGGUA--UGCUCGCGCgCGa-ACGGC- -5' |
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221 | 3' | -56.5 | AC_000008.1 | + | 15173 | 0.68 | 0.415138 |
Target: 5'- ---gCCAcACGGGCugaggagaaGCGCGCUgagGCCGa -3' miRNA: 3'- guugGGUaUGCUCG---------CGCGCGAa--CGGC- -5' |
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221 | 3' | -56.5 | AC_000008.1 | + | 4692 | 0.68 | 0.386871 |
Target: 5'- -uGCCCAUuuUGAGCGCaaGCaUGCCa -3' miRNA: 3'- guUGGGUAu-GCUCGCGcgCGaACGGc -5' |
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221 | 3' | -56.5 | AC_000008.1 | + | 19644 | 0.69 | 0.359026 |
Target: 5'- uCGGCCgGUGCGAcGUGCGCGgUUaagcaggGCCGc -3' miRNA: 3'- -GUUGGgUAUGCU-CGCGCGCgAA-------CGGC- -5' |
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221 | 3' | -56.5 | AC_000008.1 | + | 12684 | 0.69 | 0.334273 |
Target: 5'- gAGCCC--ACGGGCuGCGCGUUgugGCUGc -3' miRNA: 3'- gUUGGGuaUGCUCG-CGCGCGAa--CGGC- -5' |
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221 | 3' | -56.5 | AC_000008.1 | + | 23672 | 0.7 | 0.310005 |
Target: 5'- -cGCCgCcgACGuugccAGCGCGCGCgggUGCCa -3' miRNA: 3'- guUGG-GuaUGC-----UCGCGCGCGa--ACGGc -5' |
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221 | 3' | -56.5 | AC_000008.1 | + | 32228 | 0.71 | 0.265554 |
Target: 5'- cCGGCCCAUGgGGGCaUGCGCgUUGUCa -3' miRNA: 3'- -GUUGGGUAUgCUCGcGCGCG-AACGGc -5' |
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221 | 3' | -56.5 | AC_000008.1 | + | 5464 | 0.72 | 0.232575 |
Target: 5'- cCAGCCCcuccgcgGCGuggcccuuGGCGCGCaGCUUGCCc -3' miRNA: 3'- -GUUGGGua-----UGC--------UCGCGCG-CGAACGGc -5' |
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221 | 3' | -56.5 | AC_000008.1 | + | 12323 | 0.72 | 0.220379 |
Target: 5'- gGACCCggcgGUGCGGGCG-GCGCUgcagaGCCa -3' miRNA: 3'- gUUGGG----UAUGCUCGCgCGCGAa----CGGc -5' |
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221 | 3' | -56.5 | AC_000008.1 | + | 29227 | 1.07 | 0.000553 |
Target: 5'- uCAACCCAUACGAGCGCGCGCUUGCCGc -3' miRNA: 3'- -GUUGGGUAUGCUCGCGCGCGAACGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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