Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
221 | 5' | -54.8 | AC_000008.1 | + | 13985 | 0.66 | 0.602598 |
Target: 5'- -aCAGCAGcGUcCUGGAUUUGgGAGGGa -3' miRNA: 3'- ccGUCGUC-CGcGAUCUGAACgUUCCU- -5' |
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221 | 5' | -54.8 | AC_000008.1 | + | 9285 | 0.66 | 0.595696 |
Target: 5'- aGCGGCGGGCGaaggAGAuguugcccccacaguCUUGCAAGc- -3' miRNA: 3'- cCGUCGUCCGCga--UCU---------------GAACGUUCcu -5' |
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221 | 5' | -54.8 | AC_000008.1 | + | 8934 | 0.66 | 0.579642 |
Target: 5'- uGGCGGCgAGGuCGUUGGAaaUGCGGGc- -3' miRNA: 3'- -CCGUCG-UCC-GCGAUCUgaACGUUCcu -5' |
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221 | 5' | -54.8 | AC_000008.1 | + | 17332 | 0.66 | 0.568231 |
Target: 5'- uGGCAGCacAGGUuucuGCUGGGUgucgGCGGGGGg -3' miRNA: 3'- -CCGUCG--UCCG----CGAUCUGaa--CGUUCCU- -5' |
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221 | 5' | -54.8 | AC_000008.1 | + | 9747 | 0.66 | 0.545589 |
Target: 5'- cGGCAGCGGGCGgCggucGGGgUUGUuucuggcgGAGGu -3' miRNA: 3'- -CCGUCGUCCGC-Ga---UCUgAACG--------UUCCu -5' |
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221 | 5' | -54.8 | AC_000008.1 | + | 18459 | 0.66 | 0.545589 |
Target: 5'- cGGgGGUAGGCGCaaUGGAaggaUGUAGGGc -3' miRNA: 3'- -CCgUCGUCCGCG--AUCUga--ACGUUCCu -5' |
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221 | 5' | -54.8 | AC_000008.1 | + | 8605 | 0.67 | 0.523245 |
Target: 5'- aGGUAGgGGGgGCUccGGACccGCcGGGAg -3' miRNA: 3'- -CCGUCgUCCgCGA--UCUGaaCGuUCCU- -5' |
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221 | 5' | -54.8 | AC_000008.1 | + | 12810 | 0.67 | 0.523245 |
Target: 5'- gGGCAGCGcGCGCUgaaggagcAGuCUaGCAAGGc -3' miRNA: 3'- -CCGUCGUcCGCGA--------UCuGAaCGUUCCu -5' |
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221 | 5' | -54.8 | AC_000008.1 | + | 18224 | 0.67 | 0.501261 |
Target: 5'- aGGCGGCAGGUGa-GGGCcauaucUGCAAGa- -3' miRNA: 3'- -CCGUCGUCCGCgaUCUGa-----ACGUUCcu -5' |
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221 | 5' | -54.8 | AC_000008.1 | + | 2163 | 0.67 | 0.501261 |
Target: 5'- aGCAGCAGGaggaaGCcAGGCggcgGCGgcAGGAg -3' miRNA: 3'- cCGUCGUCCg----CGaUCUGaa--CGU--UCCU- -5' |
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221 | 5' | -54.8 | AC_000008.1 | + | 5252 | 0.67 | 0.490422 |
Target: 5'- cGGCAGUAgucGGUGCUcguccAGACggGCcAGGGu -3' miRNA: 3'- -CCGUCGU---CCGCGA-----UCUGaaCGuUCCU- -5' |
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221 | 5' | -54.8 | AC_000008.1 | + | 11777 | 0.68 | 0.469081 |
Target: 5'- aGGUGGUAGGCGCguuGGCg-GCAucgguGGAg -3' miRNA: 3'- -CCGUCGUCCGCGau-CUGaaCGUu----CCU- -5' |
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221 | 5' | -54.8 | AC_000008.1 | + | 22144 | 0.68 | 0.437991 |
Target: 5'- -cCAGCAGGCGC-GGGCggguggUGCGAGu- -3' miRNA: 3'- ccGUCGUCCGCGaUCUGa-----ACGUUCcu -5' |
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221 | 5' | -54.8 | AC_000008.1 | + | 8473 | 0.68 | 0.437991 |
Target: 5'- uGGUGGC-GGCGUcgauGGCUUGCAAGaGGc -3' miRNA: 3'- -CCGUCGuCCGCGau--CUGAACGUUC-CU- -5' |
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221 | 5' | -54.8 | AC_000008.1 | + | 17409 | 0.7 | 0.335001 |
Target: 5'- cGGUAGCcauGGGCGCgaugGGACgggUGguGGGc -3' miRNA: 3'- -CCGUCG---UCCGCGa---UCUGa--ACguUCCu -5' |
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221 | 5' | -54.8 | AC_000008.1 | + | 17140 | 0.71 | 0.318284 |
Target: 5'- aGCAGguGGCGCcgGGACUgggcgUGCAGaccguGGAc -3' miRNA: 3'- cCGUCguCCGCGa-UCUGA-----ACGUU-----CCU- -5' |
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221 | 5' | -54.8 | AC_000008.1 | + | 6580 | 0.71 | 0.302202 |
Target: 5'- gGGCAGCAGGCGCgcgucgaagUAGucuauCUUGCAu--- -3' miRNA: 3'- -CCGUCGUCCGCG---------AUCu----GAACGUuccu -5' |
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221 | 5' | -54.8 | AC_000008.1 | + | 17274 | 0.73 | 0.218765 |
Target: 5'- uGGCGGCGcGGCGCagGGACgcgcgGCuAGGAc -3' miRNA: 3'- -CCGUCGU-CCGCGa-UCUGaa---CGuUCCU- -5' |
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221 | 5' | -54.8 | AC_000008.1 | + | 23707 | 0.73 | 0.218765 |
Target: 5'- uGGUgauGGCGGGCGCUcGGGCUUGgGAGa- -3' miRNA: 3'- -CCG---UCGUCCGCGA-UCUGAACgUUCcu -5' |
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221 | 5' | -54.8 | AC_000008.1 | + | 8532 | 0.77 | 0.116397 |
Target: 5'- cGCGGCGGGCGgUGGGCc-GCGGGGGu -3' miRNA: 3'- cCGUCGUCCGCgAUCUGaaCGUUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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