miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22100 5' -55.7 NC_004927.1 + 5091 0.66 0.76965
Target:  5'- aCGGCuCCU--GCCGCugguGGUUCGUCg -3'
miRNA:   3'- -GCCG-GGAuuUGGUGuau-CCGGGCAGa -5'
22100 5' -55.7 NC_004927.1 + 2543 0.66 0.749584
Target:  5'- gCGGCCUUuacuuGCCACuacugggacgAUGGGuucCCCGUCg -3'
miRNA:   3'- -GCCGGGAuu---UGGUG----------UAUCC---GGGCAGa -5'
22100 5' -55.7 NC_004927.1 + 12087 0.66 0.749584
Target:  5'- uCGGUUCcGGGCCACGaacGGCUCGUCc -3'
miRNA:   3'- -GCCGGGaUUUGGUGUau-CCGGGCAGa -5'
22100 5' -55.7 NC_004927.1 + 26317 0.67 0.718654
Target:  5'- aCGGCUgauaCUGAACguCGUAGaGCCaCGUCa -3'
miRNA:   3'- -GCCGG----GAUUUGguGUAUC-CGG-GCAGa -5'
22100 5' -55.7 NC_004927.1 + 33221 0.72 0.418655
Target:  5'- aGGCCgUUGAACgccgggaaCACGUAGGCcCCGUCg -3'
miRNA:   3'- gCCGG-GAUUUG--------GUGUAUCCG-GGCAGa -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.