Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22102 | 3' | -53.6 | NC_004927.1 | + | 69797 | 0.66 | 0.894505 |
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Target: 5'- uAGAGUcggCG-GGUgGGGAGgucaaGUCCCg -3' miRNA: 3'- cUUUCGaa-GCuCCAgCCCUCa----CAGGG- -5' |
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| 22102 | 3' | -53.6 | NC_004927.1 | + | 32362 | 0.66 | 0.894505 |
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Target: 5'- --cGGCUcaaUCuc-GUCGGGAGUGaCCCg -3' miRNA: 3'- cuuUCGA---AGcucCAGCCCUCACaGGG- -5' |
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| 22102 | 3' | -53.6 | NC_004927.1 | + | 73636 | 0.67 | 0.848099 |
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Target: 5'- cGGGGCcgCGAGGUCGGcGGUucagCCCu -3' miRNA: 3'- cUUUCGaaGCUCCAGCCcUCAca--GGG- -5' |
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| 22102 | 3' | -53.6 | NC_004927.1 | + | 8210 | 0.67 | 0.839552 |
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Target: 5'- aGAGGCUUCGGGucGUCGGGGucgGUCa- -3' miRNA: 3'- cUUUCGAAGCUC--CAGCCCUca-CAGgg -5' |
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| 22102 | 3' | -53.6 | NC_004927.1 | + | 25214 | 0.67 | 0.829907 |
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Target: 5'- aGAGGGCggCGuAGGUCGcGGGGaaguagucgauaaUGUCCa -3' miRNA: 3'- -CUUUCGaaGC-UCCAGC-CCUC-------------ACAGGg -5' |
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| 22102 | 3' | -53.6 | NC_004927.1 | + | 21604 | 0.68 | 0.803341 |
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Target: 5'- --cGGcCUUCGAGGUCGucacGGAGUucgagagcGUCCUc -3' miRNA: 3'- cuuUC-GAAGCUCCAGC----CCUCA--------CAGGG- -5' |
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| 22102 | 3' | -53.6 | NC_004927.1 | + | 13545 | 0.69 | 0.712735 |
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Target: 5'- ---cGUUUCGGGGgcCGGGAGUcucguUCCCa -3' miRNA: 3'- cuuuCGAAGCUCCa-GCCCUCAc----AGGG- -5' |
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| 22102 | 3' | -53.6 | NC_004927.1 | + | 20452 | 0.74 | 0.459274 |
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Target: 5'- ---cGCUUCGAGGUCGccGGAG-GUCUg -3' miRNA: 3'- cuuuCGAAGCUCCAGC--CCUCaCAGGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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