Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22103 | 5' | -48.5 | NC_004927.1 | + | 17804 | 0.66 | 0.988065 |
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Target: 5'- -cUCGCCAcuucuccgagaaGAACUCGGGuucGUAcuuCCa -3' miRNA: 3'- aaAGCGGU------------UUUGAGCCCugaCAUu--GG- -5' |
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| 22103 | 5' | -48.5 | NC_004927.1 | + | 27731 | 0.66 | 0.984441 |
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Target: 5'- ---gGCCGAAugUUGGGGuCUGaaaAACCc -3' miRNA: 3'- aaagCGGUUUugAGCCCU-GACa--UUGG- -5' |
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| 22103 | 5' | -48.5 | NC_004927.1 | + | 71750 | 0.67 | 0.982342 |
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Target: 5'- -gUCGgCAGAACcCGGcGGCgGUGGCUg -3' miRNA: 3'- aaAGCgGUUUUGaGCC-CUGaCAUUGG- -5' |
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| 22103 | 5' | -48.5 | NC_004927.1 | + | 32037 | 0.67 | 0.971779 |
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Target: 5'- gUUCGCCcGAACaguuuggcuuUCGGGG-UGUAAUCu -3' miRNA: 3'- aAAGCGGuUUUG----------AGCCCUgACAUUGG- -5' |
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| 22103 | 5' | -48.5 | NC_004927.1 | + | 46091 | 0.69 | 0.948412 |
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Target: 5'- gUUCGCCGcucGCaCGGGGCU-UGACCc -3' miRNA: 3'- aAAGCGGUuu-UGaGCCCUGAcAUUGG- -5' |
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| 22103 | 5' | -48.5 | NC_004927.1 | + | 70241 | 0.69 | 0.932946 |
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Target: 5'- -aUCGUCAcgGGACUCGGGAUUccGGCa -3' miRNA: 3'- aaAGCGGU--UUUGAGCCCUGAcaUUGg -5' |
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| 22103 | 5' | -48.5 | NC_004927.1 | + | 40263 | 0.69 | 0.932946 |
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Target: 5'- aUUCGCCGcuACUucCGcuACUGUAACCa -3' miRNA: 3'- aAAGCGGUuuUGA--GCccUGACAUUGG- -5' |
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| 22103 | 5' | -48.5 | NC_004927.1 | + | 18335 | 0.69 | 0.932386 |
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Target: 5'- -gUCGUCAAGugUCccguugaGGGA-UGUGACCa -3' miRNA: 3'- aaAGCGGUUUugAG-------CCCUgACAUUGG- -5' |
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| 22103 | 5' | -48.5 | NC_004927.1 | + | 8772 | 0.7 | 0.921171 |
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Target: 5'- -cUCGUCAAucACUCGGcagaUGUGACCg -3' miRNA: 3'- aaAGCGGUUu-UGAGCCcug-ACAUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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