Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22105 | 5' | -50.6 | NC_004927.1 | + | 58890 | 0.74 | 0.640193 |
Target: 5'- gGGuaguGCCGACCUCGAcUUuGAGGUUGa -3' miRNA: 3'- gCUu---CGGCUGGAGCUuAAcUUCCAGC- -5' |
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22105 | 5' | -50.6 | NC_004927.1 | + | 60569 | 0.73 | 0.717527 |
Target: 5'- uGAAGgCGACUUCGAGgauacugauuucgaGGAGGUCGa -3' miRNA: 3'- gCUUCgGCUGGAGCUUaa------------CUUCCAGC- -5' |
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22105 | 5' | -50.6 | NC_004927.1 | + | 4578 | 0.7 | 0.844294 |
Target: 5'- uCGAGGUCGGCUUCGcuu---AGGUCGa -3' miRNA: 3'- -GCUUCGGCUGGAGCuuaacuUCCAGC- -5' |
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22105 | 5' | -50.6 | NC_004927.1 | + | 52720 | 0.68 | 0.924589 |
Target: 5'- gCGAGGUugaaGACCUCugguuucaGGAUUGGAGGUUc -3' miRNA: 3'- -GCUUCGg---CUGGAG--------CUUAACUUCCAGc -5' |
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22105 | 5' | -50.6 | NC_004927.1 | + | 68732 | 0.68 | 0.930283 |
Target: 5'- aCGAAGCCaacccCCUCGAugUGggGGaguccUCGg -3' miRNA: 3'- -GCUUCGGcu---GGAGCUuaACuuCC-----AGC- -5' |
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22105 | 5' | -50.6 | NC_004927.1 | + | 42855 | 0.67 | 0.954222 |
Target: 5'- uCGAGGCCGACgaugUGGggUGucguacaGAGGUCGg -3' miRNA: 3'- -GCUUCGGCUGga--GCUuaAC-------UUCCAGC- -5' |
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22105 | 5' | -50.6 | NC_004927.1 | + | 45867 | 0.67 | 0.953798 |
Target: 5'- gGAGGaaaaCGACCUCGAuguggacgccgUGAAGGagGa -3' miRNA: 3'- gCUUCg---GCUGGAGCUua---------ACUUCCagC- -5' |
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22105 | 5' | -50.6 | NC_004927.1 | + | 58214 | 0.67 | 0.945714 |
Target: 5'- aCGAuGGgCGACCUCGA--UGAuauugccagucAGGUCa -3' miRNA: 3'- -GCU-UCgGCUGGAGCUuaACU-----------UCCAGc -5' |
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22105 | 5' | -50.6 | NC_004927.1 | + | 67481 | 0.66 | 0.960265 |
Target: 5'- uCGAAGCUGACCccggCGAGUgggagaagcugagcGAGGG-CGa -3' miRNA: 3'- -GCUUCGGCUGGa---GCUUAa-------------CUUCCaGC- -5' |
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22105 | 5' | -50.6 | NC_004927.1 | + | 68124 | 0.66 | 0.962521 |
Target: 5'- cCGAAGCCGcucucCCUCugcUUGAGGGUg- -3' miRNA: 3'- -GCUUCGGCu----GGAGcuuAACUUCCAgc -5' |
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22105 | 5' | -50.6 | NC_004927.1 | + | 64732 | 0.66 | 0.966079 |
Target: 5'- -cGAGCCGGgUggcCGGAUUGAGGGaaUCGa -3' miRNA: 3'- gcUUCGGCUgGa--GCUUAACUUCC--AGC- -5' |
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22105 | 5' | -50.6 | NC_004927.1 | + | 58769 | 0.66 | 0.969393 |
Target: 5'- uGggGCgGuguguCCUCGGAUagagauucGggGGUCGc -3' miRNA: 3'- gCuuCGgCu----GGAGCUUAa-------CuuCCAGC- -5' |
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22105 | 5' | -50.6 | NC_004927.1 | + | 74450 | 0.66 | 0.969393 |
Target: 5'- cCGAguAGCCcGCCUCGA--UGAuGGUgGg -3' miRNA: 3'- -GCU--UCGGcUGGAGCUuaACUuCCAgC- -5' |
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22105 | 5' | -50.6 | NC_004927.1 | + | 4622 | 0.66 | 0.972469 |
Target: 5'- cCGAAGCCuGCCg-GAGUcggUGGAGGUa- -3' miRNA: 3'- -GCUUCGGcUGGagCUUA---ACUUCCAgc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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