Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22109 | 3' | -56.2 | NC_004927.1 | + | 21273 | 0.69 | 0.592296 |
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Target: 5'- --cAUCCGACUcGucGCCgGGCGACCg -3' miRNA: 3'- uguUGGGCUGA-CuuCGGgCUGCUGGg -5' |
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| 22109 | 3' | -56.2 | NC_004927.1 | + | 15074 | 0.69 | 0.61237 |
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Target: 5'- --cGCCCGugUucggagcGAAGCCCGccGCGACUg -3' miRNA: 3'- uguUGGGCugA-------CUUCGGGC--UGCUGGg -5' |
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| 22109 | 3' | -56.2 | NC_004927.1 | + | 75822 | 0.69 | 0.613428 |
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Target: 5'- gACGGCCCGcGC-GggGCaggggcaggggCCGAcCGGCCCg -3' miRNA: 3'- -UGUUGGGC-UGaCuuCG-----------GGCU-GCUGGG- -5' |
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| 22109 | 3' | -56.2 | NC_004927.1 | + | 305 | 0.69 | 0.613428 |
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Target: 5'- gACGGCCCGcGC-GggGCaggggcaggggCCGAcCGGCCCg -3' miRNA: 3'- -UGUUGGGC-UGaCuuCG-----------GGCU-GCUGGG- -5' |
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| 22109 | 3' | -56.2 | NC_004927.1 | + | 69356 | 0.66 | 0.788001 |
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Target: 5'- gACAGCCCGA----AGCCgGuuuCGACCg -3' miRNA: 3'- -UGUUGGGCUgacuUCGGgCu--GCUGGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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