Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22109 | 5' | -55.6 | NC_004927.1 | + | 70751 | 0.66 | 0.787777 |
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Target: 5'- aUGGA--GCUGGUC-GUUGAagacgagcaguuCCGACCg -3' miRNA: 3'- cACCUucCGGCCAGuCAACU------------GGCUGG- -5' |
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| 22109 | 5' | -55.6 | NC_004927.1 | + | 75253 | 0.66 | 0.768366 |
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Target: 5'- cGUGGAGGGagucuCGGUCGGggagcUGGUCGAgCCa -3' miRNA: 3'- -CACCUUCCg----GCCAGUCa----ACUGGCU-GG- -5' |
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| 22109 | 5' | -55.6 | NC_004927.1 | + | 709 | 0.67 | 0.707293 |
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Target: 5'- -gGGGAGuauaucccCCGGcccauuUCAGggGGCCGACCg -3' miRNA: 3'- caCCUUCc-------GGCC------AGUCaaCUGGCUGG- -5' |
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| 22109 | 5' | -55.6 | NC_004927.1 | + | 75481 | 0.69 | 0.611652 |
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Target: 5'- cGUGGGAGaGCCGGUCuGGUucaucuggUGugUggGGCCg -3' miRNA: 3'- -CACCUUC-CGGCCAG-UCA--------ACugG--CUGG- -5' |
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| 22109 | 5' | -55.6 | NC_004927.1 | + | 466 | 0.71 | 0.517217 |
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Target: 5'- -aGGggGGCCGGagAGacgGACgGACg -3' miRNA: 3'- caCCuuCCGGCCagUCaa-CUGgCUGg -5' |
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| 22109 | 5' | -55.6 | NC_004927.1 | + | 37833 | 0.71 | 0.507043 |
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Target: 5'- -cGGAGGGCUGGUCGGcuu-UCGACa -3' miRNA: 3'- caCCUUCCGGCCAGUCaacuGGCUGg -5' |
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| 22109 | 5' | -55.6 | NC_004927.1 | + | 15307 | 0.72 | 0.429141 |
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Target: 5'- cGUGGuu-GCCGGUCuuaucgguGUUGACCG-CCa -3' miRNA: 3'- -CACCuucCGGCCAGu-------CAACUGGCuGG- -5' |
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| 22109 | 5' | -55.6 | NC_004927.1 | + | 5137 | 0.76 | 0.265158 |
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Target: 5'- -aGGAgccaagcuuagcuugGGGCCGGUCGGUUcuUCGACCa -3' miRNA: 3'- caCCU---------------UCCGGCCAGUCAAcuGGCUGG- -5' |
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| 22109 | 5' | -55.6 | NC_004927.1 | + | 8215 | 0.79 | 0.178763 |
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Target: 5'- cGUGGAgAGGCUucgggucgucggGGUCGGUcaacUGACCGGCCu -3' miRNA: 3'- -CACCU-UCCGG------------CCAGUCA----ACUGGCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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