Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22110 | 5' | -53.8 | NC_004927.1 | + | 7994 | 0.67 | 0.839445 |
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Target: 5'- uGGUGGUgCuGUCCACaGAGgaugaacuUCGCCGUGu -3' miRNA: 3'- -CCAUUA-GuCGGGUG-CUC--------AGCGGCACu -5' |
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| 22110 | 5' | -53.8 | NC_004927.1 | + | 54795 | 0.68 | 0.774498 |
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Target: 5'- cGGUGaacgGUCAGCCCGuaguuCGAGUCG--GUGAc -3' miRNA: 3'- -CCAU----UAGUCGGGU-----GCUCAGCggCACU- -5' |
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| 22110 | 5' | -53.8 | NC_004927.1 | + | 63793 | 0.69 | 0.73405 |
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Target: 5'- --aGAUaCAGCuuCUACG-GUCGCCGUGAc -3' miRNA: 3'- ccaUUA-GUCG--GGUGCuCAGCGGCACU- -5' |
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| 22110 | 5' | -53.8 | NC_004927.1 | + | 12592 | 0.69 | 0.72783 |
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Target: 5'- uGUAAUCGGCUCGgGAguacgcuucguagagGUCGCCGUc- -3' miRNA: 3'- cCAUUAGUCGGGUgCU---------------CAGCGGCAcu -5' |
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| 22110 | 5' | -53.8 | NC_004927.1 | + | 32511 | 0.69 | 0.71214 |
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Target: 5'- aGGUcGAUgaAGCCCACGuacucgucgguguAGUUGUCGUGAg -3' miRNA: 3'- -CCA-UUAg-UCGGGUGC-------------UCAGCGGCACU- -5' |
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| 22110 | 5' | -53.8 | NC_004927.1 | + | 42513 | 0.71 | 0.63832 |
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Target: 5'- cGGcugAAUCGGU-CGCGGGUCGCCG-GAc -3' miRNA: 3'- -CCa--UUAGUCGgGUGCUCAGCGGCaCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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