Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22112 | 3' | -53.8 | NC_004927.1 | + | 52845 | 0.66 | 0.859456 |
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Target: 5'- -gCUCCgGCGGCGCUCcGGUgaaGGACg -3' miRNA: 3'- ugGAGGgUGUUGUGAGuCCGa--CUUGg -5' |
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| 22112 | 3' | -53.8 | NC_004927.1 | + | 6361 | 0.66 | 0.851153 |
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Target: 5'- cAUCUCCCACuuCcCUCGGGaguaggGAACg -3' miRNA: 3'- -UGGAGGGUGuuGuGAGUCCga----CUUGg -5' |
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| 22112 | 3' | -53.8 | NC_004927.1 | + | 54067 | 0.66 | 0.832991 |
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Target: 5'- gGCCUaCUgACGGCACUCAucGGCgUGAcaagacaGCCu -3' miRNA: 3'- -UGGA-GGgUGUUGUGAGU--CCG-ACU-------UGG- -5' |
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| 22112 | 3' | -53.8 | NC_004927.1 | + | 45963 | 0.67 | 0.824921 |
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Target: 5'- aGCCUCCUACAACGu---GGC-GAACUu -3' miRNA: 3'- -UGGAGGGUGUUGUgaguCCGaCUUGG- -5' |
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| 22112 | 3' | -53.8 | NC_004927.1 | + | 64475 | 0.67 | 0.815766 |
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Target: 5'- -aCUCCCGaauCGC-CGGGgaGAGCCu -3' miRNA: 3'- ugGAGGGUguuGUGaGUCCgaCUUGG- -5' |
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| 22112 | 3' | -53.8 | NC_004927.1 | + | 33518 | 0.67 | 0.806421 |
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Target: 5'- gACUUCuCCuCGGCGCUCAGGgaGucguCCu -3' miRNA: 3'- -UGGAG-GGuGUUGUGAGUCCgaCuu--GG- -5' |
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| 22112 | 3' | -53.8 | NC_004927.1 | + | 14586 | 0.67 | 0.806421 |
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Target: 5'- aGCgUUCCACGGCGCUCguGGGUcGccAGCCg -3' miRNA: 3'- -UGgAGGGUGUUGUGAG--UCCGaC--UUGG- -5' |
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| 22112 | 3' | -53.8 | NC_004927.1 | + | 27787 | 0.68 | 0.757207 |
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Target: 5'- cACCgCCCu--GCGCUCGGGCcaguuUGAACa -3' miRNA: 3'- -UGGaGGGuguUGUGAGUCCG-----ACUUGg -5' |
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| 22112 | 3' | -53.8 | NC_004927.1 | + | 71929 | 0.69 | 0.715506 |
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Target: 5'- -aCUCCUACGGC-CUCAGGCgguACa -3' miRNA: 3'- ugGAGGGUGUUGuGAGUCCGacuUGg -5' |
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| 22112 | 3' | -53.8 | NC_004927.1 | + | 45731 | 0.69 | 0.694124 |
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Target: 5'- uCCUCUgaACAGCAC--GGGCUGAugCa -3' miRNA: 3'- uGGAGGg-UGUUGUGagUCCGACUugG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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