Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22114 | 3' | -61.1 | NC_004927.1 | + | 63315 | 0.66 | 0.520028 |
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Target: 5'- aCGGGguuuUGGcaAGGCCGUggGCGACCuCCGGu- -3' miRNA: 3'- -GCUC----ACC--UCCGGCA--CGCUGG-GGCUga -5' |
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| 22114 | 3' | -61.1 | NC_004927.1 | + | 68608 | 0.66 | 0.490816 |
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Target: 5'- uGAGaUGGAGGCUGa-CGACCuugCCGACc -3' miRNA: 3'- gCUC-ACCUCCGGCacGCUGG---GGCUGa -5' |
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| 22114 | 3' | -61.1 | NC_004927.1 | + | 58907 | 0.67 | 0.47177 |
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Target: 5'- aCGAGUGcGAacgacgugGGuaGUGcCGACCUCGACUu -3' miRNA: 3'- -GCUCAC-CU--------CCggCAC-GCUGGGGCUGA- -5' |
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| 22114 | 3' | -61.1 | NC_004927.1 | + | 18501 | 0.67 | 0.462388 |
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Target: 5'- aCGAGUcgGGAGGCCaccGCGACCUCcucacucuauuaGACg -3' miRNA: 3'- -GCUCA--CCUCCGGca-CGCUGGGG------------CUGa -5' |
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| 22114 | 3' | -61.1 | NC_004927.1 | + | 70654 | 0.68 | 0.39966 |
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Target: 5'- aCGcGcUGGAGccGCCGgguggugGUGGCCCCGACg -3' miRNA: 3'- -GCuC-ACCUC--CGGCa------CGCUGGGGCUGa -5' |
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| 22114 | 3' | -61.1 | NC_004927.1 | + | 62175 | 0.72 | 0.246922 |
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Target: 5'- uGAGUgGGAGGCCGUGgGAaacguuccaCCCgGAUg -3' miRNA: 3'- gCUCA-CCUCCGGCACgCU---------GGGgCUGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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