miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22115 5' -55.1 NC_004927.1 + 31426 0.66 0.823293
Target:  5'- aCAAGCUGGACGUucGGCgCUGgUguuagCGCGu -3'
miRNA:   3'- cGUUCGGCCUGUA--UCG-GAUgGa----GCGC- -5'
22115 5' -55.1 NC_004927.1 + 46491 0.66 0.795519
Target:  5'- aCAAGCUggaGGACGUAGCCagcggcgACCUUuCGa -3'
miRNA:   3'- cGUUCGG---CCUGUAUCGGa------UGGAGcGC- -5'
22115 5' -55.1 NC_004927.1 + 29094 0.68 0.704488
Target:  5'- aGCGGGCCGGugucuauguCGUAGUUcucgACCUCGgGc -3'
miRNA:   3'- -CGUUCGGCCu--------GUAUCGGa---UGGAGCgC- -5'
22115 5' -55.1 NC_004927.1 + 27167 0.68 0.672534
Target:  5'- uGCGaaguAGCCGGAgu--GCUUGCCgUCGCGu -3'
miRNA:   3'- -CGU----UCGGCCUguauCGGAUGG-AGCGC- -5'
22115 5' -55.1 NC_004927.1 + 61867 0.68 0.661796
Target:  5'- ---cGUCGGACA-GGCUgACCUCGUGa -3'
miRNA:   3'- cguuCGGCCUGUaUCGGaUGGAGCGC- -5'
22115 5' -55.1 NC_004927.1 + 57704 0.71 0.502549
Target:  5'- uGCuGGUCGGugGUGGa-UACCUCGCu -3'
miRNA:   3'- -CGuUCGGCCugUAUCggAUGGAGCGc -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.