Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
22115 | 5' | -55.1 | NC_004927.1 | + | 31426 | 0.66 | 0.823293 |
Target: 5'- aCAAGCUGGACGUucGGCgCUGgUguuagCGCGu -3' miRNA: 3'- cGUUCGGCCUGUA--UCG-GAUgGa----GCGC- -5' |
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22115 | 5' | -55.1 | NC_004927.1 | + | 46491 | 0.66 | 0.795519 |
Target: 5'- aCAAGCUggaGGACGUAGCCagcggcgACCUUuCGa -3' miRNA: 3'- cGUUCGG---CCUGUAUCGGa------UGGAGcGC- -5' |
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22115 | 5' | -55.1 | NC_004927.1 | + | 29094 | 0.68 | 0.704488 |
Target: 5'- aGCGGGCCGGugucuauguCGUAGUUcucgACCUCGgGc -3' miRNA: 3'- -CGUUCGGCCu--------GUAUCGGa---UGGAGCgC- -5' |
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22115 | 5' | -55.1 | NC_004927.1 | + | 27167 | 0.68 | 0.672534 |
Target: 5'- uGCGaaguAGCCGGAgu--GCUUGCCgUCGCGu -3' miRNA: 3'- -CGU----UCGGCCUguauCGGAUGG-AGCGC- -5' |
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22115 | 5' | -55.1 | NC_004927.1 | + | 61867 | 0.68 | 0.661796 |
Target: 5'- ---cGUCGGACA-GGCUgACCUCGUGa -3' miRNA: 3'- cguuCGGCCUGUaUCGGaUGGAGCGC- -5' |
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22115 | 5' | -55.1 | NC_004927.1 | + | 57704 | 0.71 | 0.502549 |
Target: 5'- uGCuGGUCGGugGUGGa-UACCUCGCu -3' miRNA: 3'- -CGuUCGGCCugUAUCggAUGGAGCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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