Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22116 | 3' | -46.3 | NC_004927.1 | + | 43211 | 0.66 | 0.999014 |
Target: 5'- aAGUUGUGGAUgcuggCGUUCAggCgGGCCg -3' miRNA: 3'- cUUAGCAUUUGa----GCGAGUaaGgCUGG- -5' |
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22116 | 3' | -46.3 | NC_004927.1 | + | 43009 | 0.66 | 0.999014 |
Target: 5'- ---cUGUcAGCUcaCGCUCGUUCCGAa- -3' miRNA: 3'- cuuaGCAuUUGA--GCGAGUAAGGCUgg -5' |
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22116 | 3' | -46.3 | NC_004927.1 | + | 49280 | 0.66 | 0.998949 |
Target: 5'- gGggUCGUGggUUcuucuaagcgaggaCGCUC---CCGACCa -3' miRNA: 3'- -CuuAGCAUuuGA--------------GCGAGuaaGGCUGG- -5' |
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22116 | 3' | -46.3 | NC_004927.1 | + | 14273 | 0.67 | 0.997792 |
Target: 5'- --cUCGUAGuucACucauaggcugaaUCGCUCGUUCC-ACCg -3' miRNA: 3'- cuuAGCAUU---UG------------AGCGAGUAAGGcUGG- -5' |
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22116 | 3' | -46.3 | NC_004927.1 | + | 36048 | 0.67 | 0.99734 |
Target: 5'- --uUCGUGAACUUGCUCAagUCUauUCa -3' miRNA: 3'- cuuAGCAUUUGAGCGAGUa-AGGcuGG- -5' |
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22116 | 3' | -46.3 | NC_004927.1 | + | 16110 | 0.67 | 0.996812 |
Target: 5'- --uUCGUGAGCgcCGCUgAUUCUGucaACCu -3' miRNA: 3'- cuuAGCAUUUGa-GCGAgUAAGGC---UGG- -5' |
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22116 | 3' | -46.3 | NC_004927.1 | + | 12378 | 0.67 | 0.996202 |
Target: 5'- --uUCGauGAgUCGUUCAUcggcUCCGGCCc -3' miRNA: 3'- cuuAGCauUUgAGCGAGUA----AGGCUGG- -5' |
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22116 | 3' | -46.3 | NC_004927.1 | + | 74432 | 0.68 | 0.994692 |
Target: 5'- uGAUgGUGGGCaCGCUCAUUCguGCCg -3' miRNA: 3'- cUUAgCAUUUGaGCGAGUAAGgcUGG- -5' |
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22116 | 3' | -46.3 | NC_004927.1 | + | 70244 | 0.68 | 0.991553 |
Target: 5'- aGAAUCGUcacgGGACUCGgg-AUUCCGGCa -3' miRNA: 3'- -CUUAGCA----UUUGAGCgagUAAGGCUGg -5' |
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22116 | 3' | -46.3 | NC_004927.1 | + | 45039 | 0.69 | 0.988748 |
Target: 5'- ---gCGUGAACguacuUCGCUCGaaguUUCCGuACCu -3' miRNA: 3'- cuuaGCAUUUG-----AGCGAGU----AAGGC-UGG- -5' |
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22116 | 3' | -46.3 | NC_004927.1 | + | 22135 | 0.69 | 0.983248 |
Target: 5'- ---cCGU-AGCUgGCUCGUaCUGACCg -3' miRNA: 3'- cuuaGCAuUUGAgCGAGUAaGGCUGG- -5' |
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22116 | 3' | -46.3 | NC_004927.1 | + | 39449 | 0.7 | 0.972109 |
Target: 5'- ------cAAGCUCGUUCAggcgauacuccuucUUCCGACCa -3' miRNA: 3'- cuuagcaUUUGAGCGAGU--------------AAGGCUGG- -5' |
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22116 | 3' | -46.3 | NC_004927.1 | + | 25106 | 0.71 | 0.969885 |
Target: 5'- gGAuUCGUccgaguGgUCGUUCAUUCCGACg -3' miRNA: 3'- -CUuAGCAuu----UgAGCGAGUAAGGCUGg -5' |
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22116 | 3' | -46.3 | NC_004927.1 | + | 23881 | 0.72 | 0.935054 |
Target: 5'- --cUCGgugAAGCUCGUUCAU-CCGAgCg -3' miRNA: 3'- cuuAGCa--UUUGAGCGAGUAaGGCUgG- -5' |
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22116 | 3' | -46.3 | NC_004927.1 | + | 7327 | 0.75 | 0.85701 |
Target: 5'- uGAGUCGUGGACUCGauaacuacCUCAg--CGACCu -3' miRNA: 3'- -CUUAGCAUUUGAGC--------GAGUaagGCUGG- -5' |
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22116 | 3' | -46.3 | NC_004927.1 | + | 69108 | 0.78 | 0.694294 |
Target: 5'- gGAGcCGUAuauGCUCGgUCAcUCCGACCg -3' miRNA: 3'- -CUUaGCAUu--UGAGCgAGUaAGGCUGG- -5' |
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22116 | 3' | -46.3 | NC_004927.1 | + | 45087 | 0.79 | 0.671746 |
Target: 5'- gGAAUCGUcuGCUC-CUCAccgcuuuucauaUUCCGACCg -3' miRNA: 3'- -CUUAGCAuuUGAGcGAGU------------AAGGCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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