Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22117 | 3' | -51.7 | NC_004927.1 | + | 46906 | 0.66 | 0.959954 |
Target: 5'- -cGUGACGccuuUGACGACgGCGAggGCg -3' miRNA: 3'- guCAUUGCu---GCUGCUGgCGCUgaUGg -5' |
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22117 | 3' | -51.7 | NC_004927.1 | + | 46472 | 0.66 | 0.956437 |
Target: 5'- ---cAGCGGCGACcuuucgacucgggaGGCCGUggagugguacuucgaGACUGCCg -3' miRNA: 3'- gucaUUGCUGCUG--------------CUGGCG---------------CUGAUGG- -5' |
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22117 | 3' | -51.7 | NC_004927.1 | + | 67433 | 0.66 | 0.956034 |
Target: 5'- cCGG-AACGACccguGCGAaggaCGaCGACUACCu -3' miRNA: 3'- -GUCaUUGCUGc---UGCUg---GC-GCUGAUGG- -5' |
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22117 | 3' | -51.7 | NC_004927.1 | + | 64333 | 0.66 | 0.953561 |
Target: 5'- gCAGUAuuuCGACGugGACauuccgaGCgguugggagcggcccGACUACUa -3' miRNA: 3'- -GUCAUu--GCUGCugCUGg------CG---------------CUGAUGG- -5' |
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22117 | 3' | -51.7 | NC_004927.1 | + | 59817 | 0.66 | 0.951861 |
Target: 5'- cCGGUGACGACGAcuCGAUUGauAUUGCg -3' miRNA: 3'- -GUCAUUGCUGCU--GCUGGCgcUGAUGg -5' |
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22117 | 3' | -51.7 | NC_004927.1 | + | 66747 | 0.66 | 0.951861 |
Target: 5'- uCGGUGucuCGAUGACGAacCCGCucGCcGCCg -3' miRNA: 3'- -GUCAUu--GCUGCUGCU--GGCGc-UGaUGG- -5' |
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22117 | 3' | -51.7 | NC_004927.1 | + | 34520 | 0.66 | 0.946972 |
Target: 5'- gCAG-AACG-CGGCGAguccgguCUGCGACUGuCCc -3' miRNA: 3'- -GUCaUUGCuGCUGCU-------GGCGCUGAU-GG- -5' |
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22117 | 3' | -51.7 | NC_004927.1 | + | 3595 | 0.66 | 0.942738 |
Target: 5'- uCGGgu-CGAUGACcACCGUGAUgagcugGCCg -3' miRNA: 3'- -GUCauuGCUGCUGcUGGCGCUGa-----UGG- -5' |
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22117 | 3' | -51.7 | NC_004927.1 | + | 53763 | 0.67 | 0.932558 |
Target: 5'- gAGUAAC-ACGAcCGGCgGUGAgaACCg -3' miRNA: 3'- gUCAUUGcUGCU-GCUGgCGCUgaUGG- -5' |
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22117 | 3' | -51.7 | NC_004927.1 | + | 64277 | 0.67 | 0.915284 |
Target: 5'- gAGUGgacuACGACGGCGAgCCGCuACgggaauccgACCu -3' miRNA: 3'- gUCAU----UGCUGCUGCU-GGCGcUGa--------UGG- -5' |
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22117 | 3' | -51.7 | NC_004927.1 | + | 57886 | 0.68 | 0.908991 |
Target: 5'- -cGUGACGAUGGCGAagguUCGCG-UUGCg -3' miRNA: 3'- guCAUUGCUGCUGCU----GGCGCuGAUGg -5' |
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22117 | 3' | -51.7 | NC_004927.1 | + | 52998 | 0.68 | 0.881212 |
Target: 5'- --aUGACGACGAUGAUgGCGggaauuggaaACUGCUc -3' miRNA: 3'- gucAUUGCUGCUGCUGgCGC----------UGAUGG- -5' |
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22117 | 3' | -51.7 | NC_004927.1 | + | 61429 | 0.68 | 0.880466 |
Target: 5'- -cGUGACGGCGAaucaggcaaugguCGAUggCGCGGCUGgCg -3' miRNA: 3'- guCAUUGCUGCU-------------GCUG--GCGCUGAUgG- -5' |
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22117 | 3' | -51.7 | NC_004927.1 | + | 67800 | 0.69 | 0.865816 |
Target: 5'- ---gGGCGGCGGCGugaCGCaGACUGCUc -3' miRNA: 3'- gucaUUGCUGCUGCug-GCG-CUGAUGG- -5' |
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22117 | 3' | -51.7 | NC_004927.1 | + | 60032 | 0.69 | 0.849477 |
Target: 5'- aCAGUAACGACGuuaacaucuCGAagcuCCGCGAaCUGgCu -3' miRNA: 3'- -GUCAUUGCUGCu--------GCU----GGCGCU-GAUgG- -5' |
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22117 | 3' | -51.7 | NC_004927.1 | + | 44956 | 0.69 | 0.840972 |
Target: 5'- gCAGUAACGGCGAacCGGCCaucgGACUucuCCu -3' miRNA: 3'- -GUCAUUGCUGCU--GCUGGcg--CUGAu--GG- -5' |
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22117 | 3' | -51.7 | NC_004927.1 | + | 61471 | 0.71 | 0.773032 |
Target: 5'- gGGUAGCacgGGCGacucgguggacuacGCGACCGCcGACUACg -3' miRNA: 3'- gUCAUUG---CUGC--------------UGCUGGCG-CUGAUGg -5' |
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22117 | 3' | -51.7 | NC_004927.1 | + | 15065 | 0.71 | 0.745786 |
Target: 5'- uCGG-AGCGAaGcCcGCCGCGACUGCCg -3' miRNA: 3'- -GUCaUUGCUgCuGcUGGCGCUGAUGG- -5' |
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22117 | 3' | -51.7 | NC_004927.1 | + | 49506 | 0.72 | 0.725068 |
Target: 5'- gUAGUGACGACGAUuGGCaagaaggucuaCGCGGCUGgCu -3' miRNA: 3'- -GUCAUUGCUGCUG-CUG-----------GCGCUGAUgG- -5' |
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22117 | 3' | -51.7 | NC_004927.1 | + | 63870 | 0.72 | 0.725068 |
Target: 5'- cCGGUuACGACGACu-CCGCugguGAUUACCu -3' miRNA: 3'- -GUCAuUGCUGCUGcuGGCG----CUGAUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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