miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22118 3' -65.6 NC_004927.1 + 61748 0.67 0.304159
Target:  5'- uCGAUGCUCUCaCCGaG-GGGGCCuacCCGg -3'
miRNA:   3'- -GCUGCGAGGGaGGC-CaCCCCGGc--GGC- -5'
22118 3' -65.6 NC_004927.1 + 7572 0.67 0.304159
Target:  5'- cCGGCGUUCCauucaaCCGa--GGGCCGCCGc -3'
miRNA:   3'- -GCUGCGAGGga----GGCcacCCCGGCGGC- -5'
22118 3' -65.6 NC_004927.1 + 35191 0.68 0.253219
Target:  5'- gGGCgucgGCUCCCgugaaguccUCGGcGGGGCCGUCGu -3'
miRNA:   3'- gCUG----CGAGGGa--------GGCCaCCCCGGCGGC- -5'
22118 3' -65.6 NC_004927.1 + 61917 0.69 0.219785
Target:  5'- uCGGCGCUCUccgucaCUUCGaGUGGGGgCGCUc -3'
miRNA:   3'- -GCUGCGAGG------GAGGC-CACCCCgGCGGc -5'
22118 3' -65.6 NC_004927.1 + 10385 0.75 0.078494
Target:  5'- aCGACGC-CCCa-CGGUGGGGCguuuCGCCGu -3'
miRNA:   3'- -GCUGCGaGGGagGCCACCCCG----GCGGC- -5'
22118 3' -65.6 NC_004927.1 + 22211 0.78 0.051617
Target:  5'- uCGAUGaCUUCCUCCGGgucgucaacGGGGUCGCCGu -3'
miRNA:   3'- -GCUGC-GAGGGAGGCCa--------CCCCGGCGGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.