Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
22118 | 3' | -65.6 | NC_004927.1 | + | 61748 | 0.67 | 0.304159 |
Target: 5'- uCGAUGCUCUCaCCGaG-GGGGCCuacCCGg -3' miRNA: 3'- -GCUGCGAGGGaGGC-CaCCCCGGc--GGC- -5' |
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22118 | 3' | -65.6 | NC_004927.1 | + | 7572 | 0.67 | 0.304159 |
Target: 5'- cCGGCGUUCCauucaaCCGa--GGGCCGCCGc -3' miRNA: 3'- -GCUGCGAGGga----GGCcacCCCGGCGGC- -5' |
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22118 | 3' | -65.6 | NC_004927.1 | + | 35191 | 0.68 | 0.253219 |
Target: 5'- gGGCgucgGCUCCCgugaaguccUCGGcGGGGCCGUCGu -3' miRNA: 3'- gCUG----CGAGGGa--------GGCCaCCCCGGCGGC- -5' |
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22118 | 3' | -65.6 | NC_004927.1 | + | 61917 | 0.69 | 0.219785 |
Target: 5'- uCGGCGCUCUccgucaCUUCGaGUGGGGgCGCUc -3' miRNA: 3'- -GCUGCGAGG------GAGGC-CACCCCgGCGGc -5' |
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22118 | 3' | -65.6 | NC_004927.1 | + | 10385 | 0.75 | 0.078494 |
Target: 5'- aCGACGC-CCCa-CGGUGGGGCguuuCGCCGu -3' miRNA: 3'- -GCUGCGaGGGagGCCACCCCG----GCGGC- -5' |
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22118 | 3' | -65.6 | NC_004927.1 | + | 22211 | 0.78 | 0.051617 |
Target: 5'- uCGAUGaCUUCCUCCGGgucgucaacGGGGUCGCCGu -3' miRNA: 3'- -GCUGC-GAGGGAGGCCa--------CCCCGGCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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