miRNA: 3'- aaGCUa-----CUUG-GUUGGCCGGGCCuu---CA- -5'
miRNA: 3'- aagCUACUUGgUugGCCGGGCCu------UCA- -5'
miRNA: 3'- aAGCUaCUUGGuuGGCCGGGCCUUca -5'
miRNA: 3'- aagCUa---CUUGGUUGGCCGGGCcUUCa -5'
miRNA: 3'- aagCUa---CUUGGUUGGCCGGGCcUUCa -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22118 | 5' | -55.2 | NC_004927.1 | + | 53692 | 0.68 | 0.642558 |
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Target: 5'- -cCGAggguucGAGCgCGACCGGCCCGGcucuuGUa -3' miRNA: 3'- aaGCUa-----CUUG-GUUGGCCGGGCCuu---CA- -5' |
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| 22118 | 5' | -55.2 | NC_004927.1 | + | 51111 | 0.69 | 0.599135 |
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Target: 5'- --aGGUGGACgAugCGGCCCGGugauacuGGUc -3' miRNA: 3'- aagCUACUUGgUugGCCGGGCCu------UCA- -5' |
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| 22118 | 5' | -55.2 | NC_004927.1 | + | 2882 | 0.7 | 0.566823 |
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Target: 5'- aUCGG-GAACCu-CCGGCUCGGGAa- -3' miRNA: 3'- aAGCUaCUUGGuuGGCCGGGCCUUca -5' |
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| 22118 | 5' | -55.2 | NC_004927.1 | + | 75804 | 0.72 | 0.434383 |
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Target: 5'- --gGGgcagGGGCCGACCGGCCCGaGAGa -3' miRNA: 3'- aagCUa---CUUGGUUGGCCGGGCcUUCa -5' |
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| 22118 | 5' | -55.2 | NC_004927.1 | + | 287 | 0.72 | 0.434383 |
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Target: 5'- --gGGgcagGGGCCGACCGGCCCGaGAGa -3' miRNA: 3'- aagCUa---CUUGGUUGGCCGGGCcUUCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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