Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
22119 | 3' | -47.7 | NC_004927.1 | + | 26164 | 0.66 | 0.996916 |
Target: 5'- cCCGGucaACUc-CCUGCGuGUGguAGUGCCc -3' miRNA: 3'- -GGUC---UGAaaGGAUGC-CGCguUUAUGG- -5' |
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22119 | 3' | -47.7 | NC_004927.1 | + | 31309 | 0.66 | 0.99633 |
Target: 5'- --cGACUUUCCg--GGCGCAG--GCUc -3' miRNA: 3'- gguCUGAAAGGaugCCGCGUUuaUGG- -5' |
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22119 | 3' | -47.7 | NC_004927.1 | + | 46263 | 0.67 | 0.989181 |
Target: 5'- aCCAGAUgggcguccCCUACGGCacGCAGGacuCCu -3' miRNA: 3'- -GGUCUGaaa-----GGAUGCCG--CGUUUau-GG- -5' |
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22119 | 3' | -47.7 | NC_004927.1 | + | 64670 | 0.7 | 0.960561 |
Target: 5'- aCCGG-CgugCUUACGGCGCG-AUGCg -3' miRNA: 3'- -GGUCuGaaaGGAUGCCGCGUuUAUGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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