Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22120 | 3' | -52.6 | NC_004927.1 | + | 5024 | 0.66 | 0.918261 |
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Target: 5'- cGCGUugcuuucgUCGCCgcuuccacucucGGUAUCGCUcUCGUCGg -3' miRNA: 3'- cUGCA--------AGCGG------------CCAUAGUGAuAGCGGU- -5' |
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| 22120 | 3' | -52.6 | NC_004927.1 | + | 71822 | 0.66 | 0.912026 |
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Target: 5'- cGCGUcgguaUCuCCGGUAUCACgagGUCGUgAa -3' miRNA: 3'- cUGCA-----AGcGGCCAUAGUGa--UAGCGgU- -5' |
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| 22120 | 3' | -52.6 | NC_004927.1 | + | 21915 | 0.67 | 0.884399 |
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Target: 5'- cGACGUUCuCCGGguucgucUCACccguguUCGCCAu -3' miRNA: 3'- -CUGCAAGcGGCCau-----AGUGau----AGCGGU- -5' |
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| 22120 | 3' | -52.6 | NC_004927.1 | + | 58557 | 0.67 | 0.852686 |
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Target: 5'- uGCGUUCGCuguucaggcgucCGGUGUUGCUAccucgaugcUCGCUAu -3' miRNA: 3'- cUGCAAGCG------------GCCAUAGUGAU---------AGCGGU- -5' |
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| 22120 | 3' | -52.6 | NC_004927.1 | + | 2725 | 0.68 | 0.826474 |
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Target: 5'- aGACcgUCuUCGGUGUCACUAcCGCCGu -3' miRNA: 3'- -CUGcaAGcGGCCAUAGUGAUaGCGGU- -5' |
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| 22120 | 3' | -52.6 | NC_004927.1 | + | 10369 | 0.69 | 0.798439 |
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Target: 5'- gGGCGuUUCGCCGu--UUACUcUCGCCAc -3' miRNA: 3'- -CUGC-AAGCGGCcauAGUGAuAGCGGU- -5' |
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| 22120 | 3' | -52.6 | NC_004927.1 | + | 15310 | 0.69 | 0.758699 |
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Target: 5'- cGACGUggUUGCCGGUcuuAUCGgUGUugacCGCCAu -3' miRNA: 3'- -CUGCA--AGCGGCCA---UAGUgAUA----GCGGU- -5' |
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| 22120 | 3' | -52.6 | NC_004927.1 | + | 71784 | 0.72 | 0.607028 |
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Target: 5'- cGCGUUCucgaacagGCCGGUAUCACUccaucgaGUCGgCAg -3' miRNA: 3'- cUGCAAG--------CGGCCAUAGUGA-------UAGCgGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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