Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22122 | 3' | -57.6 | NC_004927.1 | + | 70865 | 0.66 | 0.710957 |
Target: 5'- --aUCGAGCagacggagGAAGauauugCGCCGGuCGCCc -3' miRNA: 3'- gcaAGCUCGa-------CUUCg-----GCGGCCuGCGG- -5' |
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22122 | 3' | -57.6 | NC_004927.1 | + | 32590 | 0.66 | 0.690341 |
Target: 5'- --aUCGAGUccGAGGUCGCUcgGGugGCUc -3' miRNA: 3'- gcaAGCUCGa-CUUCGGCGG--CCugCGG- -5' |
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22122 | 3' | -57.6 | NC_004927.1 | + | 33721 | 0.66 | 0.690341 |
Target: 5'- aGUUCGAGUcGguGUCGCCauAUGCCa -3' miRNA: 3'- gCAAGCUCGaCuuCGGCGGccUGCGG- -5' |
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22122 | 3' | -57.6 | NC_004927.1 | + | 33233 | 0.67 | 0.627569 |
Target: 5'- aCGUUCuccUUGAGGCCGuuGaACGCCg -3' miRNA: 3'- -GCAAGcucGACUUCGGCggCcUGCGG- -5' |
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22122 | 3' | -57.6 | NC_004927.1 | + | 47531 | 0.67 | 0.617069 |
Target: 5'- uGUUcCGAcgagucGCUGAGaacGUCGCCGGA-GCCg -3' miRNA: 3'- gCAA-GCU------CGACUU---CGGCGGCCUgCGG- -5' |
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22122 | 3' | -57.6 | NC_004927.1 | + | 23067 | 0.68 | 0.596112 |
Target: 5'- cCGUUCGGGCc---GUCGUCGGACGa- -3' miRNA: 3'- -GCAAGCUCGacuuCGGCGGCCUGCgg -5' |
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22122 | 3' | -57.6 | NC_004927.1 | + | 42515 | 0.69 | 0.524013 |
Target: 5'- --cUCG-GCUGAaucGGUCGCgGGuCGCCg -3' miRNA: 3'- gcaAGCuCGACU---UCGGCGgCCuGCGG- -5' |
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22122 | 3' | -57.6 | NC_004927.1 | + | 15068 | 0.75 | 0.257481 |
Target: 5'- uGUUCgGAGC-GAAGcCCGCCGcGACuGCCg -3' miRNA: 3'- gCAAG-CUCGaCUUC-GGCGGC-CUG-CGG- -5' |
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22122 | 3' | -57.6 | NC_004927.1 | + | 20463 | 0.75 | 0.257481 |
Target: 5'- gGUUCGAgagucGCUucGAGGUCGCCGGAgGUCu -3' miRNA: 3'- gCAAGCU-----CGA--CUUCGGCGGCCUgCGG- -5' |
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22122 | 3' | -57.6 | NC_004927.1 | + | 70657 | 0.77 | 0.190011 |
Target: 5'- aGUaCGcGCUGGAGCCGCCgggugguGGugGCCc -3' miRNA: 3'- gCAaGCuCGACUUCGGCGG-------CCugCGG- -5' |
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22122 | 3' | -57.6 | NC_004927.1 | + | 21805 | 0.79 | 0.139314 |
Target: 5'- cCGagCGAgccGCUGGAGCCGCUGGAacCGCCc -3' miRNA: 3'- -GCaaGCU---CGACUUCGGCGGCCU--GCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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