miRNA display CGI


Results 1 - 12 of 12 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22123 3' -44.4 NC_004927.1 + 14655 0.66 0.999804
Target:  5'- --cGCucCGUCUUCGAGGAugggauagucGGGGUCg -3'
miRNA:   3'- uuaUGuuGUAGGAGCUUCUu---------UUCCAG- -5'
22123 3' -44.4 NC_004927.1 + 23620 0.66 0.999666
Target:  5'- --cGCAAUAUCgUCGggGAgugccugccGAGcGGUCu -3'
miRNA:   3'- uuaUGUUGUAGgAGCuuCU---------UUU-CCAG- -5'
22123 3' -44.4 NC_004927.1 + 58766 0.66 0.999666
Target:  5'- --gGCggUguGUCCUCGGauAGAGAuucggGGGUCg -3'
miRNA:   3'- uuaUGuuG--UAGGAGCU--UCUUU-----UCCAG- -5'
22123 3' -44.4 NC_004927.1 + 52737 0.67 0.999123
Target:  5'- --gGCGAcCAUCCUCGGGugcGAGGUUg -3'
miRNA:   3'- uuaUGUU-GUAGGAGCUUcuuUUCCAG- -5'
22123 3' -44.4 NC_004927.1 + 20150 0.68 0.99864
Target:  5'- --aGCAcucGCAUCCcCGAuaccaucGggGAGGUCg -3'
miRNA:   3'- uuaUGU---UGUAGGaGCUu------CuuUUCCAG- -5'
22123 3' -44.4 NC_004927.1 + 24656 0.69 0.996377
Target:  5'- --gACGACcgcUCCUUGAuaGGAAcAGGUCg -3'
miRNA:   3'- uuaUGUUGu--AGGAGCU--UCUUuUCCAG- -5'
22123 3' -44.4 NC_004927.1 + 55991 0.7 0.990288
Target:  5'- --cGCAACAUCUUgGAcuuccgcauGGAGGGGGUUg -3'
miRNA:   3'- uuaUGUUGUAGGAgCU---------UCUUUUCCAG- -5'
22123 3' -44.4 NC_004927.1 + 73460 0.71 0.978103
Target:  5'- gAGUAUAGCGUCUcCGAGGAc--GGUCa -3'
miRNA:   3'- -UUAUGUUGUAGGaGCUUCUuuuCCAG- -5'
22123 3' -44.4 NC_004927.1 + 50900 0.72 0.975289
Target:  5'- cAGUACGACAaCCucaccgUCGAGGAGGGGGcCa -3'
miRNA:   3'- -UUAUGUUGUaGG------AGCUUCUUUUCCaG- -5'
22123 3' -44.4 NC_004927.1 + 62875 0.72 0.968885
Target:  5'- ---cCGACGUUCUUGggGAAguuagguucGAGGUCa -3'
miRNA:   3'- uuauGUUGUAGGAGCuuCUU---------UUCCAG- -5'
22123 3' -44.4 NC_004927.1 + 67871 0.72 0.968885
Target:  5'- --cACGGCAUcuaCCUCGAAGAcGAGGa- -3'
miRNA:   3'- uuaUGUUGUA---GGAGCUUCUuUUCCag -5'
22123 3' -44.4 NC_004927.1 + 61758 0.73 0.952704
Target:  5'- --cGCAACAUCCUCGAugcucucaccGAGGGGGcCu -3'
miRNA:   3'- uuaUGUUGUAGGAGCUu---------CUUUUCCaG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.