miRNA display CGI


Results 1 - 14 of 14 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22124 3' -58.2 NC_004927.1 + 20414 0.66 0.689071
Target:  5'- uGCUGGAGuguUGAUGggguucacgaCGugGUUCGGCu -3'
miRNA:   3'- gCGGCCUCu--GCUACg---------GCugCGAGUCG- -5'
22124 3' -58.2 NC_004927.1 + 36934 0.66 0.678712
Target:  5'- -aCCGGAGACucaGCgGGCuGUUCAGCc -3'
miRNA:   3'- gcGGCCUCUGcuaCGgCUG-CGAGUCG- -5'
22124 3' -58.2 NC_004927.1 + 69346 0.67 0.626501
Target:  5'- aGCCGGuuuCGA--CCGACGCUCAu- -3'
miRNA:   3'- gCGGCCucuGCUacGGCUGCGAGUcg -5'
22124 3' -58.2 NC_004927.1 + 52874 0.67 0.626501
Target:  5'- uCGCUGGAGGagcuUGCUGGaaugGUUCAGCu -3'
miRNA:   3'- -GCGGCCUCUgcu-ACGGCUg---CGAGUCG- -5'
22124 3' -58.2 NC_004927.1 + 50751 0.67 0.616038
Target:  5'- uGgCGGuGAUGGUGgCGAUGCU-GGCg -3'
miRNA:   3'- gCgGCCuCUGCUACgGCUGCGAgUCG- -5'
22124 3' -58.2 NC_004927.1 + 43230 0.68 0.573356
Target:  5'- aGCCGcgaaguauuccccGAaguuGugGAUGCUGGCGUUCAGg -3'
miRNA:   3'- gCGGC-------------CU----CugCUACGGCUGCGAGUCg -5'
22124 3' -58.2 NC_004927.1 + 50394 0.68 0.553794
Target:  5'- uGCCGGGaGCGGuggugguggUGCUGGCGgUCAGa -3'
miRNA:   3'- gCGGCCUcUGCU---------ACGGCUGCgAGUCg -5'
22124 3' -58.2 NC_004927.1 + 73638 0.68 0.553794
Target:  5'- -aCCGGGGccGCGAgGUCGGCGgUUCAGCc -3'
miRNA:   3'- gcGGCCUC--UGCUaCGGCUGC-GAGUCG- -5'
22124 3' -58.2 NC_004927.1 + 59375 0.68 0.533423
Target:  5'- gCGUCGGcGGCGGUaaugGUCGGCGCUCucggAGUg -3'
miRNA:   3'- -GCGGCCuCUGCUA----CGGCUGCGAG----UCG- -5'
22124 3' -58.2 NC_004927.1 + 61041 0.68 0.533423
Target:  5'- cCGCUGGccGACGAgagUGaaGGCGCUCGGa -3'
miRNA:   3'- -GCGGCCu-CUGCU---ACggCUGCGAGUCg -5'
22124 3' -58.2 NC_004927.1 + 23027 0.69 0.493544
Target:  5'- uGCCGGAGACGua-CCGGCGaauccgUCAcGCu -3'
miRNA:   3'- gCGGCCUCUGCuacGGCUGCg-----AGU-CG- -5'
22124 3' -58.2 NC_004927.1 + 20233 0.69 0.464552
Target:  5'- uCGCCGGGGACGGacuUGCCacgGAgGUcgaacacgUCGGCc -3'
miRNA:   3'- -GCGGCCUCUGCU---ACGG---CUgCG--------AGUCG- -5'
22124 3' -58.2 NC_004927.1 + 72820 0.7 0.436473
Target:  5'- uCGCCucuGAGAgCGAUGCUGACGUUgAuGCg -3'
miRNA:   3'- -GCGGc--CUCU-GCUACGGCUGCGAgU-CG- -5'
22124 3' -58.2 NC_004927.1 + 59240 0.7 0.418306
Target:  5'- gGCCGGGGACGuuAUGgCGAaGUUgGGCg -3'
miRNA:   3'- gCGGCCUCUGC--UACgGCUgCGAgUCG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.