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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22124 | 5' | -55.5 | NC_004927.1 | + | 52841 | 0.66 | 0.800287 |
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Target: 5'- cGGCGG--CGCUCcGGUGAAgGAcGCg -3' miRNA: 3'- aCCGCCuuGCGAGcCUACUUgCU-CGa -5' |
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| 22124 | 5' | -55.5 | NC_004927.1 | + | 24520 | 0.66 | 0.790789 |
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Target: 5'- gUGGCGuauucGGAUGC-CGGAcGAgACGAGCg -3' miRNA: 3'- -ACCGC-----CUUGCGaGCCUaCU-UGCUCGa -5' |
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| 22124 | 5' | -55.5 | NC_004927.1 | + | 58443 | 0.66 | 0.765381 |
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Target: 5'- gUGGCGGAACGCUCaGGcucagcucucccaacUGAaggaagaccuguACGAGaCg -3' miRNA: 3'- -ACCGCCUUGCGAGcCU---------------ACU------------UGCUC-Ga -5' |
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| 22124 | 5' | -55.5 | NC_004927.1 | + | 57088 | 0.68 | 0.678198 |
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Target: 5'- gGGCGGGauggagugcuACGUgaaGGGUGGggacguugGCGAGCUa -3' miRNA: 3'- aCCGCCU----------UGCGag-CCUACU--------UGCUCGA- -5' |
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| 22124 | 5' | -55.5 | NC_004927.1 | + | 71300 | 0.73 | 0.393258 |
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Target: 5'- cGGaGGAGCGCgaCGGuUGGGCGGGCUu -3' miRNA: 3'- aCCgCCUUGCGa-GCCuACUUGCUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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