Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22126 | 3' | -55 | NC_004927.1 | + | 13168 | 0.66 | 0.796204 |
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Target: 5'- aCGGCCCggacuuaaccagaacGCGACCguaUCCU-UCGAGg -3' miRNA: 3'- cGCCGGG---------------CGCUGGacaAGGAuAGUUC- -5' |
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| 22126 | 3' | -55 | NC_004927.1 | + | 47546 | 0.69 | 0.658678 |
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Target: 5'- aGgGGgCUGaCGACCUGUUCCga-CGAGu -3' miRNA: 3'- -CgCCgGGC-GCUGGACAAGGauaGUUC- -5' |
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| 22126 | 3' | -55 | NC_004927.1 | + | 66802 | 0.7 | 0.604933 |
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Target: 5'- gGUGGcCCCGCGACCgag-CCgggCGAGa -3' miRNA: 3'- -CGCC-GGGCGCUGGacaaGGauaGUUC- -5' |
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| 22126 | 3' | -55 | NC_004927.1 | + | 66018 | 0.71 | 0.551732 |
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Target: 5'- gGUGGCCCucgaaagcGCGACCaccccgUGUUCCUAUuccccCGAGa -3' miRNA: 3'- -CGCCGGG--------CGCUGG------ACAAGGAUA-----GUUC- -5' |
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| 22126 | 3' | -55 | NC_004927.1 | + | 67536 | 0.71 | 0.510214 |
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Target: 5'- cCGuuCCGCGACCUGUUCgUGgagCAGGu -3' miRNA: 3'- cGCcgGGCGCUGGACAAGgAUa--GUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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