Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22127 | 5' | -51.4 | NC_004927.1 | + | 72108 | 0.66 | 0.928028 |
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Target: 5'- cGAGCAggACGUGGAACCCGUccggGGCGa -3' miRNA: 3'- -CUUGU--UGUGCCUUGGGCGauagUCGC- -5' |
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| 22127 | 5' | -51.4 | NC_004927.1 | + | 29498 | 0.67 | 0.902972 |
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Target: 5'- gGGACGGuCGUGGAACCaGCaGUCGGCGu -3' miRNA: 3'- -CUUGUU-GUGCCUUGGgCGaUAGUCGC- -5' |
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| 22127 | 5' | -51.4 | NC_004927.1 | + | 40218 | 0.68 | 0.865554 |
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Target: 5'- -uACGAUugGGAACCCGaccucgaacUCAGCc -3' miRNA: 3'- cuUGUUGugCCUUGGGCgau------AGUCGc -5' |
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| 22127 | 5' | -51.4 | NC_004927.1 | + | 33856 | 0.68 | 0.857305 |
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Target: 5'- cGAAcCGGCACGGGucggucggcauCCCGUacUCGGCGg -3' miRNA: 3'- -CUU-GUUGUGCCUu----------GGGCGauAGUCGC- -5' |
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| 22127 | 5' | -51.4 | NC_004927.1 | + | 72394 | 0.69 | 0.831161 |
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Target: 5'- cGGGCGACcCGGAcUCCGCUcgUGGUGa -3' miRNA: 3'- -CUUGUUGuGCCUuGGGCGAuaGUCGC- -5' |
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| 22127 | 5' | -51.4 | NC_004927.1 | + | 68031 | 0.7 | 0.793382 |
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Target: 5'- cGGCaAACGCacAGCCCGCUGUUGGCGu -3' miRNA: 3'- cUUG-UUGUGccUUGGGCGAUAGUCGC- -5' |
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| 22127 | 5' | -51.4 | NC_004927.1 | + | 54505 | 0.84 | 0.169528 |
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Target: 5'- uGAGCuGAUGCGGAACuCCGCUAUCGGUGa -3' miRNA: 3'- -CUUG-UUGUGCCUUG-GGCGAUAGUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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