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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22128 | 5' | -57.5 | NC_004927.1 | + | 55552 | 0.66 | 0.653672 |
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Target: 5'- cGUGGACgaggaaaccuACGAGGCCCGCAaagaGGagaACg -3' miRNA: 3'- -CACCUG----------UGUUCCGGGUGUagg-CCg--UG- -5' |
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| 22128 | 5' | -57.5 | NC_004927.1 | + | 14799 | 0.67 | 0.610753 |
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Target: 5'- --cGACGCGAGGUaggCUAUGUCCGGCu- -3' miRNA: 3'- cacCUGUGUUCCG---GGUGUAGGCCGug -5' |
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| 22128 | 5' | -57.5 | NC_004927.1 | + | 58931 | 0.67 | 0.589364 |
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Target: 5'- -cGGAUGgAAGGCgCgGgAUUCGGCACg -3' miRNA: 3'- caCCUGUgUUCCG-GgUgUAGGCCGUG- -5' |
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| 22128 | 5' | -57.5 | NC_004927.1 | + | 41049 | 0.68 | 0.547056 |
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Target: 5'- uGUGGAaACGGGGUguCCACAgaCGGCAUa -3' miRNA: 3'- -CACCUgUGUUCCG--GGUGUagGCCGUG- -5' |
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| 22128 | 5' | -57.5 | NC_004927.1 | + | 71845 | 0.68 | 0.514941 |
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Target: 5'- uGUGGucuucgaGCAUgAAGGCCCGCGucgguaucUCCGGUAUc -3' miRNA: 3'- -CACC-------UGUG-UUCCGGGUGU--------AGGCCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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