Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22129 | 3' | -51 | NC_004927.1 | + | 35533 | 0.66 | 0.955769 |
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Target: 5'- cCGAGGUuGCC-CUUG-GAGAUAGACc -3' miRNA: 3'- cGCUUCAuCGGcGAGCgUUCUAUCUG- -5' |
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| 22129 | 3' | -51 | NC_004927.1 | + | 2339 | 0.66 | 0.951452 |
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Target: 5'- gGCGguGUAGUCGgUgGCGAGGaAGAa -3' miRNA: 3'- -CGCuuCAUCGGCgAgCGUUCUaUCUg -5' |
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| 22129 | 3' | -51 | NC_004927.1 | + | 59466 | 0.66 | 0.951452 |
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Target: 5'- cGCGAA--AGCCGCgaGCAGGAgaagaAGAUg -3' miRNA: 3'- -CGCUUcaUCGGCGagCGUUCUa----UCUG- -5' |
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| 22129 | 3' | -51 | NC_004927.1 | + | 2635 | 0.66 | 0.936844 |
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Target: 5'- cCGAAGUAGUCaCUCaGUAGGGUuucgAGGCg -3' miRNA: 3'- cGCUUCAUCGGcGAG-CGUUCUA----UCUG- -5' |
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| 22129 | 3' | -51 | NC_004927.1 | + | 27130 | 0.67 | 0.925697 |
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Target: 5'- -aGAAGUucgaGGUCGC-CGCGcugaagucucAGGUAGACg -3' miRNA: 3'- cgCUUCA----UCGGCGaGCGU----------UCUAUCUG- -5' |
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| 22129 | 3' | -51 | NC_004927.1 | + | 21103 | 0.67 | 0.925697 |
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Target: 5'- uCGAAGUcGCCGCUCGCGc------- -3' miRNA: 3'- cGCUUCAuCGGCGAGCGUucuaucug -5' |
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| 22129 | 3' | -51 | NC_004927.1 | + | 46098 | 0.67 | 0.919698 |
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Target: 5'- aCGAguGGUucGCCGCUCGCAcg--GGGCu -3' miRNA: 3'- cGCU--UCAu-CGGCGAGCGUucuaUCUG- -5' |
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| 22129 | 3' | -51 | NC_004927.1 | + | 9427 | 0.69 | 0.861755 |
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Target: 5'- cCGuAGaAGCCGUuaUCGCAAuGGUAGACc -3' miRNA: 3'- cGCuUCaUCGGCG--AGCGUU-CUAUCUG- -5' |
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| 22129 | 3' | -51 | NC_004927.1 | + | 27275 | 0.7 | 0.778234 |
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Target: 5'- cGUGAGGUuGCCGUcgUCGUcaaccugagcGAGAUGGAUg -3' miRNA: 3'- -CGCUUCAuCGGCG--AGCG----------UUCUAUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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