Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22130 | 3' | -57.2 | NC_004927.1 | + | 7764 | 0.66 | 0.694729 |
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Target: 5'- cGGAaguugcuacACCGGACGCCguaGCUCcguucuucgACCGCu -3' miRNA: 3'- -CCU---------UGGCCUGCGGgc-CGAGaa-------UGGUG- -5' |
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| 22130 | 3' | -57.2 | NC_004927.1 | + | 69109 | 0.67 | 0.673814 |
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Target: 5'- aGGAGCCGuauAUGCUCGGUcacUCcgACCGCc -3' miRNA: 3'- -CCUUGGCc--UGCGGGCCG---AGaaUGGUG- -5' |
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| 22130 | 3' | -57.2 | NC_004927.1 | + | 17933 | 0.67 | 0.642194 |
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Target: 5'- cGGAagACCGG-CGagaaGGCUCcgUGCCACg -3' miRNA: 3'- -CCU--UGGCCuGCggg-CCGAGa-AUGGUG- -5' |
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| 22130 | 3' | -57.2 | NC_004927.1 | + | 35272 | 0.67 | 0.631624 |
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Target: 5'- cGAACCGGGCGUCCu-CUUUcGCCAUc -3' miRNA: 3'- cCUUGGCCUGCGGGccGAGAaUGGUG- -5' |
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| 22130 | 3' | -57.2 | NC_004927.1 | + | 5120 | 0.69 | 0.5519 |
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Target: 5'- uGGGGCCGGuCGguucuucgaccaccaUCaCGGCUCcUGCCGCu -3' miRNA: 3'- -CCUUGGCCuGC---------------GG-GCCGAGaAUGGUG- -5' |
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| 22130 | 3' | -57.2 | NC_004927.1 | + | 28412 | 0.81 | 0.10352 |
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Target: 5'- aGAGCCGGGCGUCCGGUUC---CCACa -3' miRNA: 3'- cCUUGGCCUGCGGGCCGAGaauGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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