Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22132 | 3' | -56.7 | NC_004927.1 | + | 29058 | 0.68 | 0.645218 |
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Target: 5'- gGCAUCAUCUGCAucccgAGGaCGgCCUUGcGGa -3' miRNA: 3'- -UGUAGUAGGCGU-----UCCgGCaGGAGC-CC- -5' |
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| 22132 | 3' | -56.7 | NC_004927.1 | + | 73950 | 0.68 | 0.60285 |
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Target: 5'- aACAgcUCG-CCGCAguugcggcggAGGCUGUUCUCGGu -3' miRNA: 3'- -UGU--AGUaGGCGU----------UCCGGCAGGAGCCc -5' |
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| 22132 | 3' | -56.7 | NC_004927.1 | + | 21653 | 0.69 | 0.581772 |
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Target: 5'- cGCGUCGUCgGgCAGGaaGCCGUCCUUGu- -3' miRNA: 3'- -UGUAGUAGgC-GUUC--CGGCAGGAGCcc -5' |
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| 22132 | 3' | -56.7 | NC_004927.1 | + | 58778 | 0.7 | 0.529879 |
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Target: 5'- aACGUC--CCGUggGGCggugUGUCCUCGGa -3' miRNA: 3'- -UGUAGuaGGCGuuCCG----GCAGGAGCCc -5' |
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| 22132 | 3' | -56.7 | NC_004927.1 | + | 20109 | 0.7 | 0.509583 |
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Target: 5'- -aGUCAgCCGgGuucGGGCCGUUCUCGGu -3' miRNA: 3'- ugUAGUaGGCgU---UCCGGCAGGAGCCc -5' |
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| 22132 | 3' | -56.7 | NC_004927.1 | + | 23076 | 0.71 | 0.441418 |
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Target: 5'- aACGUCccaCCGUucGGGCCGUCgUCGGa -3' miRNA: 3'- -UGUAGua-GGCGu-UCCGGCAGgAGCCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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