Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22134 | 3' | -56.6 | NC_004927.1 | + | 43019 | 0.67 | 0.720754 |
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Target: 5'- -aGGUCGAuAC-CUGUCAG-CUCaCGCu -3' miRNA: 3'- agCCAGCUuUGcGGCAGUCgGAG-GCG- -5' |
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| 22134 | 3' | -56.6 | NC_004927.1 | + | 21237 | 0.67 | 0.700178 |
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Target: 5'- uUCcGUCGAuuaccuccACGUCGUCAGCgUCCa- -3' miRNA: 3'- -AGcCAGCUu-------UGCGGCAGUCGgAGGcg -5' |
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| 22134 | 3' | -56.6 | NC_004927.1 | + | 66918 | 0.68 | 0.637299 |
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Target: 5'- -gGGUUGAGcugaugaccACGCCGUa--CCUCCGUg -3' miRNA: 3'- agCCAGCUU---------UGCGGCAgucGGAGGCG- -5' |
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| 22134 | 3' | -56.6 | NC_004927.1 | + | 75241 | 0.68 | 0.637299 |
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Target: 5'- cUCGGUCGggGaGCUgGUCgAGCCaaUCCGg -3' miRNA: 3'- -AGCCAGCuuUgCGG-CAG-UCGG--AGGCg -5' |
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| 22134 | 3' | -56.6 | NC_004927.1 | + | 29211 | 0.68 | 0.616207 |
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Target: 5'- uUCGGcuUCGuagaGCCaUCAGgCUCCGCg -3' miRNA: 3'- -AGCC--AGCuuugCGGcAGUCgGAGGCG- -5' |
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| 22134 | 3' | -56.6 | NC_004927.1 | + | 34233 | 0.71 | 0.4928 |
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Target: 5'- cUCGGggCGAAGCGCCcuUCGGCgUgCCGUc -3' miRNA: 3'- -AGCCa-GCUUUGCGGc-AGUCGgA-GGCG- -5' |
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| 22134 | 3' | -56.6 | NC_004927.1 | + | 33417 | 0.71 | 0.463611 |
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Target: 5'- aUCGaGUCGGAaguaaACGUCGUUGGCCgcuUUCGCa -3' miRNA: 3'- -AGC-CAGCUU-----UGCGGCAGUCGG---AGGCG- -5' |
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| 22134 | 3' | -56.6 | NC_004927.1 | + | 7168 | 0.71 | 0.454083 |
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Target: 5'- uUCGGUCGGGcuaucACGUCGUgGGUCUUgGUa -3' miRNA: 3'- -AGCCAGCUU-----UGCGGCAgUCGGAGgCG- -5' |
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| 22134 | 3' | -56.6 | NC_004927.1 | + | 4736 | 0.72 | 0.417076 |
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Target: 5'- cUCGGUCGAuuGCGuuGaaCGGCCUuCCGUc -3' miRNA: 3'- -AGCCAGCUu-UGCggCa-GUCGGA-GGCG- -5' |
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| 22134 | 3' | -56.6 | NC_004927.1 | + | 21338 | 0.76 | 0.237533 |
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Target: 5'- uUCGGUCGGAGCGCCGUU-GUCgUCgGUg -3' miRNA: 3'- -AGCCAGCUUUGCGGCAGuCGG-AGgCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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