miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22134 5' -45.4 NC_004927.1 + 27169 0.66 0.999159
Target:  5'- gGUGCGAAGUagCCGGAGugcuUGCCGu-- -3'
miRNA:   3'- -CGUGCUUUA--GGUCUCuau-ACGGUuuu -5'
22134 5' -45.4 NC_004927.1 + 12060 0.67 0.99711
Target:  5'- cCACGGAcuguugaacGUCCAGAGAa--GCCGAc- -3'
miRNA:   3'- cGUGCUU---------UAGGUCUCUauaCGGUUuu -5'
22134 5' -45.4 NC_004927.1 + 19251 0.67 0.996527
Target:  5'- -aACGGAGUCCAGAacGAguccgGUGUCAGc- -3'
miRNA:   3'- cgUGCUUUAGGUCU--CUa----UACGGUUuu -5'
22134 5' -45.4 NC_004927.1 + 56839 0.68 0.991993
Target:  5'- uCACGAuggacuUCCAGAGGaAUGUCAAu- -3'
miRNA:   3'- cGUGCUuu----AGGUCUCUaUACGGUUuu -5'
22134 5' -45.4 NC_004927.1 + 70594 0.73 0.928066
Target:  5'- cGCACGAGGUCgCGGAGuUGUGguucaCCAAAGa -3'
miRNA:   3'- -CGUGCUUUAG-GUCUCuAUAC-----GGUUUU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.