Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22135 | 3' | -52.8 | NC_004927.1 | + | 13060 | 0.67 | 0.866232 |
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Target: 5'- cUCUcCUcCGAauccauccaguaGGUCGggaagGGGGAGUCGGCa -3' miRNA: 3'- -AGAaGA-GCU------------UCAGCa----CCCUUCGGCUG- -5' |
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| 22135 | 3' | -52.8 | NC_004927.1 | + | 29803 | 0.67 | 0.849772 |
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Target: 5'- aCUUCUCGcAGUUGUgGGGGAGaUUGAa -3' miRNA: 3'- aGAAGAGCuUCAGCA-CCCUUC-GGCUg -5' |
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| 22135 | 3' | -52.8 | NC_004927.1 | + | 42993 | 0.68 | 0.811426 |
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Target: 5'- -gUUC-CGAAG-CGUGucuguucgguagauGGGAGCCGACg -3' miRNA: 3'- agAAGaGCUUCaGCAC--------------CCUUCGGCUG- -5' |
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| 22135 | 3' | -52.8 | NC_004927.1 | + | 50305 | 0.68 | 0.795277 |
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Target: 5'- gUCUgcUCUCGAAaUCG-GGGAGuGCCGGu -3' miRNA: 3'- -AGA--AGAGCUUcAGCaCCCUU-CGGCUg -5' |
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| 22135 | 3' | -52.8 | NC_004927.1 | + | 75549 | 0.71 | 0.637872 |
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Target: 5'- uUUUUCcagUCGggGUCGgaugGGGAggGGUCGGCu -3' miRNA: 3'- -AGAAG---AGCuuCAGCa---CCCU--UCGGCUG- -5' |
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| 22135 | 3' | -52.8 | NC_004927.1 | + | 4855 | 0.76 | 0.383259 |
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Target: 5'- uUCaUCUCGggGUCGU-GGAAGUCGGg -3' miRNA: 3'- -AGaAGAGCuuCAGCAcCCUUCGGCUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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